How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Absolute values in Leptin ELISA kit are higher than normal. Does it depend on washing procedure?

I have been using mouse leptin ELISA kit (Catalog number (90030) from crystal chem. I have been doing manual washing between my steps as I didn't have automatic washer instrument. Does this cause...

12 August 2020 4,352 4 View

I would like to ask how can i get adiponectin as drug to increase insulin sensitivity in obese people ?

Adiponectin as a drug

23 January 2020 8,571 1 View

Best companies to measure RAT hormones and HbA1c?

Good afternoon, Currently my team is performing animal studies with majority of the time using rats of different strains. Our latest experiment was a weight loss study with diet-induced obesity...

14 November 2019 7,139 2 View

Best companies to measure RAT hormones and HbA1c?

Good afternoon, Currently my team is performing animal studies with majority of the time using rats of different strains. Our latest experiment was a weight loss study with diet-induced obesity...

10 November 2019 2,033 2 View

How to calculate the AUC and cut-off in a ROC curve for an inverse association?

Hello, I am analyzing 2 parameters which are called adiponectin and 1h-insulin. There is an inverse relationship between both. Low adiponectin levels are associated with high 1h-insulin levels....

02 March 2019 6,229 5 View

How can I generate a log/log standard curve in R for ELISA analyses?

I carry out ELISA experiments, in which a log/log curve fit is required in order to calculate concentration from O.D. I have used the drc package previously to fit a 4PL model, but this kit...

25 February 2019 8,654 7 View

How can I generate a log/log standard curve in R for ELISA analyses?

I carry out ELISA experiments, in which a log/log curve fit is required in order to calculate concentration from O.D. I have used the drc package previously to fit a 4PL model, but this kit...

01 February 2019 2,938 7 View

What is more appropriate control group in db/db mice model?

We are planning to use db/db mice model in our study. According to articles heterozygous animals are used more often in comparison with WT as the control group. Could you explain what are...

21 January 2019 3,327 3 View

What is more appropriate control group in db/db mice model?

We are planning to use db/db mice model in our study. According to articles heterozygous animals are used more often in comparison with WT as the control group. Could you explain what are...

31 December 2018 2,120 3 View

From where can I find a IR graph or the structure of anti-leptin antibody?

This is mainly to identify the functional groups of the antibody to see what kind of interactions would it show with nanoparticles.

11 December 2018 6,977 0 View