Recommendation on a Transposon for Gram Positive Mutagenesis?

22 January 2018 1 6K Report

I want to create a transposon mutagenesis library for a gram positive bacterial strain. Does anyone have any suggestion/recommendation for transposons which work well in gram positive? I am planing on using horizontal gene transfer to introduce the transposon.

Andrew Michael Hogan

Hi Adam,

Our lab looks quite highly on this paper. I've given the title below. They create a Tn mutant library in S. aureus using the mariner transposon.

Multidrug Intrinsic Resistance Factors in Staphylococcus aureusIdentified by Profiling Fitness within High-Diversity Transposon Libraries

10.1128/mBio.00950-16

Hope this helps

Andrew

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