Andrew Michael Hogan

4 Questions 19 Answers 0 Followers

Questions related from Andrew Michael Hogan

Best PCR DNA Polymerase for Both High Fidelity and Long Amplicons?

Hi all, I'm doing some window shopping on supplier websites for a DNA polymerase that I can use in PCR rxns to get high fidelty amplification and good yield of long products (>12kb). My...

09 October 2019 2,286 0 View

Does formaldehyde fixation affect bacterial cell morphology/nucleoid condensation?

We work with a difficult-to-stain Gram-negative bacterium. We need to stain the membrane and nucleoid with different dyes (unfortunately, we cant use the FM series of membrane dyes from...

03 April 2019 876 2 View

Maximum size for Overlap extension PCR (SOEing PCR)?

I have a gene that I need synthesized that is about 4150 bp long. Getting it synthesized completely in one piece will be expensive and time-consuming. I've used overlap PCR before to make ~1000 bp...

06 November 2017 299 3 View

Does anyone have a protocol for staining gram negative bacteria with the membrane stain DiI-C18?

Currently, I stain with 1-5 ug/mL DiI-C18 for 30 mins at 37oC, but most of the cells aren't staining. I can't fix my cells, because my cells express GFP and I've noticed decreased GFP signal after...

01 January 1970 6,795 1 View