12 Questions 8 Answers 0 Followers
Questions related from Adam Radek Martinez
I am trying to figure out whether I can create virons with the plasmids from this paper using a Plat (Platinum) cell line is derived from 293T cells. https://www.nature.com/articles/3301447 The...
24 September 2020 2,966 3 View
Hello, I am looking to extract this protein called methanobactin using chromatography with Diaion-HP20. Its been done before and literature states the following: This is what the paper says: The...
21 March 2019 8,821 0 View
Hello Everyone, Recently I engineered a new fusion gene which contained a promoter region fused to a protein which is a hybrid of two proteins (genetically engineered in a certain fashion). It is...
30 September 2018 3,681 5 View
Hello! I am wondering whether if I use FastDigest Green in a T4 DNA ligation reaction, will it work. On Fischer's website it says that the buffer is compatible for downstream applications, but I...
20 August 2018 5,581 3 View
Bacterial biosensors (which usually contain plasmids with a reporter gene fused to a regulatory promoter) have been shown to be an alternative to traditional analytics for heavy-metal pollutant...
07 May 2018 4,661 2 View
I have been pondering the idea of using the construction of a cosmid genomic (or metagenomic) library for the identification of novel genes in gram positive bacteria. With the research I have...
04 February 2018 2,658 4 View
I want to create a transposon mutagenesis library for a gram positive bacterial strain. Does anyone have any suggestion/recommendation for transposons which work well in gram positive? I am...
22 January 2018 6,492 1 View
Hello Everyone, I am developing a system with two plasmids. One of the plasmids have been assigned the incompatibility group of IncH, the other is plasmid pK19mobsacB in which I am looking into...
02 October 2017 900 3 View
Hello, I am doing some bacterial transformations, and I need a GFP plasmid for reconstruction. I need to insert a new promotor region into the plasmid, and then heat shock it into an e. coli...
06 January 2017 7,418 3 View
Hello, I have come up with a new set of primers for the amplification of this one protein for a project. I am now writing, and I am not sure how I should describe the process in how I made them. I...
28 December 2016 5,330 2 View
Hello, When I grow two newly isolated bacterial strain which are silver resistant on a LB agar plate, I am noticing a zone of clearing around the colonies. These zones of clearing are Ag...
22 November 2016 9,383 5 View
I am studying a couple of bacteria which have the property of being silver resistant. I think that the mechanism of resistance is some type of controlled accumulation in the cytoplasm of the...
22 September 2016 6,490 1 View
