Recommendation for tritium labeling kit?

More Zophia Lim's questions See All

Generating multiple standard curves from a single template?

Hi everyone! After thinking about it on my own, I'm posting a question here. Has anyone ever tried creating qPCR standard curve samples (plasmid or DNA standard) by inserting multiple target...

30 June 2024 1,569 1 View

How to purify boronate esters?

How to purify boron esters? Hello. I'm synthesizing borates in my lab. My questions are as follows: 1. How to purify boronate esters like B(OR)3 compounds, and it is a UV undetected...

23 May 2024 7,072 2 View

Apart from the stadiometer, what is the next best measure for height among 6-10 year old children?

We are currently doing community work where we have to measure the height and weight of children 6-10 years old. The community work is done across different communities. Thus, since the...

03 April 2024 7,311 0 View

RNA QC for RNASeq - Proceed or not proceed?

Hi All, I would like to seek for your expert input for below queries on RNA QC for RNASeq: Can we proceed for RNASeq run on RNA less than 10ng, with DV200 in between 68 - 80%?Details as below:...

18 March 2024 5,936 2 View

Why is there no visible opalescent band formed after sucrose density gradient ultracentrifugation for phage purification?

I am currently conducting research on purifying Myoviridae phage particles using sucrose density gradient ultracentrifugation. However, I have encountered a challenge where no visible opalescent...

12 March 2024 9,387 3 View

What is the reason for %RSE=0 ?

hi I just start building a nonmem pharmacokinetics model. i got a problem with my estimate result .. is there any reason for %RSE=0?? if anyone know about this issue , please explain this...

04 March 2024 6,077 0 View

Does fixing cells kill intracellular proteins?

I'm following a protocol to bind fluorescent ligand to receptors expressed on cells in a microplate. The protocol includes fixing cells with 4% PFA for 20 mins. This prevents cell loss during...

09 January 2024 7,517 7 View

Cross Reference by DOI issued by ResearchGate?

Looking for somebody who had similar experience when you use DOI issued by ResearchGate. I published one paper in a conference in 2007. The conference seems not to publish their proceeding, and I...

25 December 2023 5,537 1 View

Social cognitive - the empathy of brain development and its effect ?

Required a research paper with raw data to conduct a comparison of recent research and future direction

05 December 2023 2,528 1 View

How to obtain a compact film using atomic layer deposition method?

Could I obtain a compact film through the atomic layer deposition method? If yes, why as-deposited SnO2 film with a thickness of 17 nm fail to protect the underneath layer film of my device? Could...

05 November 2023 8,153 0 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Can I use Polyjet after its expiration date?

I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?

23 July 2024 8,059 1 View

Why is there a significant edge deviation in radar point cloud and camera registration?

The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...

23 July 2024 7,479 3 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

Increasing Area Ratio on Quality control Standard on LCMS ?

Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...

08 July 2024 3,150 2 View

Is the wash in my IP the problem or something else?

The image shows 2 western blots. The first one is of protein A, and the second is for the flag tag that the protein is bound to. The lanes are as follows Ladder, Beads, SAB(unbound), and elution....

01 July 2024 3,183 0 View

How to properly label fish feed pellets with stable isotopes for tracing C and N flow?

Hi, I´d like to trace the C and N flow in a recirculating aquaculture system by labelling the fish feed with concentrated stable isotopes. From a supplier, I found a product ouf of algae lysate...

19 June 2024 746 0 View

Method to prevent crosstalk fluorescene signal between 2 dye labeled microparticles?

My goal is to see two kinds of particles mixed well in an aggregated form. My experiment was as follows: 1. Prepared Microparticles 1 + EDC/NHS + RhoB, and Microparticles 2 + EDC/NHS + FITC. 2....

19 June 2024 2,680 1 View

Please share the HNMR sample preparation procedure for isotopic labelling test to confirm source of N2 during ENRR to ammonia.?

During electrocatalytic nitrogen reduction to ammonia, we perform isotopic labelling test using HNMR to confirm source of ammonia.

29 May 2024 5,165 0 View

Can FITC fluorescence stay in conjugated proteins after running a Western-Blot?

I have FITC labeled proteins, and I would like to detect its fluorescence after running proteins western blot. Does fluorescence quench in WB condition? so I can detect FITC labeling (without...

27 May 2024 4,087 3 View