Recombinase Polymerase Amplification (RPA) technology

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What are the applicable reinforcement learning based control methods for systems that have control delay?

I am studying the experimental application of reinforcement learning (RL) based control methods. For that reason, I modeled a nonlinear system without delay and trained the RL agent. In this...

02 February 2021 7,999 3 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View

How can I produce Alpha-monopropionin and Alpha-monobutyrin in lab scale?

I understand that glycerol should be reacted with propaoiocic acid to produce monopropionin and with butyric acid to produce monobutyrin , if you can help with the reaction conditions based on 1...

13 January 2021 1,151 4 View

What is the appropriate period for indexing an article in the Scopus database?

Dear RG members The indexing of a published article in the Scopus database may depend on several factors. The issue is that my colleague and I have published separately two articles in the same...

16 December 2020 4,361 19 View

I need equations to calculate the activity of the enzyme glutamanase and basic phosphatase in soil and plants?

I need the rates for calculating the mentioned enzymes after the laboratory work. I have trouble calculating and extracting the activity of the mentioned enzymes in soil and plants because of the...

03 November 2020 8,601 1 View

How can I know that a variant is novel?

I found a variant which is not reported in gnomAD or ExAC, but still has an rs. Should I consider it as a novel variant? when can I say that ceratin variant is novel?

15 October 2020 9,539 3 View

Do you have any qualitative or quantitative methodologies for evaluating gender equality plans in HE?

I am collating evaluation methodologies. So, broader methodologies for inclusion in my report are also welcome. Please also indicate the preferred citation for the evaluation methodology.

14 October 2020 4,266 7 View

How to compare the effect of changing single amino acid in a protein in a 3D model structure using Swiss-Model?

I run sequening on a sample and found a pathogenic variant. Then I draw a 3Dprotein model on Swiss-Model for the wild type protein and the mutatnt protein. I don't know how to comment on things...

05 October 2020 579 4 View

What are the recent research direction of Skin Cancer Detection ?

I am doing a research on Skin Cancer Detection. I have read a lot of redundant research papers about the problems which related to the same topic. Therefore, I wish to know the recent research...

04 October 2020 8,628 1 View

What can I refer for Depth estimation using multiple camera input?

Currently, I am working on my final year project which involves depth estimation form multiple camera inputs for video analysis purposes. I am new in this field and struggling to look a the...

30 September 2020 3,642 3 View

Which vial septa the right one to be used for HPLC?

This is the unanswered question stored very long in my mind and till now I could not find a right answer. Google search educated me that the red surface is the PTFE material compatible to wider...

21 February 2021 546 3 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 819 4 View

How to resolve a corrupted .odb file?

Hello. I run my Abaqus simulation in the supercomputer and once it's done, I copied the result (odb file) into my local computer using winSCP. But I faced a problem to read that file (see...

08 February 2021 6,198 3 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

How to compile UDF for parallel processing ?

I am trying to use a UDF for velocity input in the room from a fan. I have created a UDF but when I am trying to load the UDF it shows an error stating that it is not compiled for parallel...

03 February 2021 2,187 3 View

Anyone knows about how to stop pH getting acidic after 7 days formulating the formulation?

i am trying to formulating one formulation but the problem i am facing is after formulating and adjusting it's pH, when i try to check pH after 7 days it is getting down towards the acidic side....

02 February 2021 9,343 3 View

How to analyse multiple Likert scales?

Hello everyone! I've encountered a problem when trying to figure out the methodology of my dissertation. The study has multiple variables and will be, in general, rather complicated, but my only...

02 February 2021 2,086 3 View

How seniors face covid 19 without fear?

Seniors in the world with diabetics and hypertension and heart diseases are millions ,and they are facing covid.therefore we should give them advices and precausions to face covid 19,this...

26 January 2021 8,349 4 View

What is the largest disadvantage in using of face masks at present? What would you like to be improved?

We all use face masks many hours a day. I wonder what are your items you like to be improved. We have the opportunity to write an application for support to a sponsor. Your answers will help us a...

18 January 2021 1,311 4 View