How to insert a simply supported boundary condition without in-plane fixation in ANSYS (Transient sructural)?

Dear all, I have a question regarding imposing a boundary condition in ANSYS (Transient Structural). The problem is a slamming of a composite plate in water (FSI problem). The geometry of the...

27 October 2020 640 3 View

Gelatin nano particles, obtained by two-step desolvation, do not re-disperse in water. What is the problem?

Hello I have synthesized gelatin type A nano-particles by two-step desolvation. After 4 days of freeze-drying, the powder was kept in the freezer (-80 C). Today I tried to redisperse the...

18 February 2020 9,494 10 View

The small dimension of gelatin nano gel. what is the problem?

Hi I am trying to Synthesis gelatin (type A) nano particles by two-step desolvation method. By DLS, the dimension of gelatin particles are less than 50 nm. I am not sure what is wrong with my...

23 July 2019 964 3 View

In MTT or Alamar Blu test for cytotoxicity assay by using UV spectrophotometery, biologists use absorbance even more than 1. Is it correct?

In MTT or Alamar Blu test for cytotoxicity assay by using UV spectrophotometery, I saw many biologist researchers that use absorbance even more than 1 for their calculations. Is it correct? As I...

10 March 2019 4,442 3 View

How can we get full text of articles which have no published PDF in the internet and have been published before 2000?

I am looking for some articles anywhere, searched internet, wrote letter to author, asked my professors for help, nevertheless couldn't get them. therefore I decide to test my chance here! Regards

05 June 2018 4,126 7 View

To make flexible polyurethane foam, necessary to observe the mixing process?

20 June 2016 8,595 7 View

How much GAGs (glycosaminoglycans) is expected of MSCs in their ECM?

Hello, Does anyone know how much GAGs I can collect if I decellularize MSCs in their stemness state and can I stain this GAGs with Alcian Blue? Thanks

23 January 2016 2,565 4 View

Infinite dilution activity coefficient from molecular dynamics simulation?

24 March 2015 8,718 1 View

What is the integrin binding site for Vitronectin?

As we know vitronectin and some other proteins can bind to different types of integrin. I want to know about the binding site in Integrin which the vitronectin with a RGD-sequence can interact...

30 January 2015 5,378 5 View

How can I extract nodal values of displacement, coordinates and time of each material point at every increment of time in ABAQUS/EXPLICIT?

How can I extract " nodal values of displacement, coordinates and time of each material point at every increment of time" in ABAQUS/EXPLICIT?

30 January 2015 2,597 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View