Raghad Mouhamad
ISO: 10260, 1992 Standard: This standard prescribes 90% (v/v) ethanol for chlorophyll extraction and measurement1. The chlorophyll contents of cultures of the cyanobacterium Synechococcus elongatus Nägeli and the chlorophyte Scenedesmus acutus Meyen were determined by means of a series of concentrations of ethanol/water mixtures which were employed as extracting agents1. The extraction yields obtained with 90% ethanol were compared to those obtained with methanol and acetone1. On the basis of the calculated error level, improvements to the ISO: 10260, 1992 standard method have been suggested1. Absolute Ethanol Extraction: This method involves adding 2~3ml of 95% ethanol to the mortar, then a little CaCO3 and quartz sand, grinding into a homogenate, then adding 10ml of absolute ethanol, continuing grinding until the tissue turns white, and standing still in the dark for 3~5min2.
Each method has its own advantages and potential limitations, and the choice between them can depend on factors such as the specific type of sample you are working with, the equipment you have available, and the specific goals of your experiment. It’s always a good idea to review the scientific literature and consult with experts in the field when choosing a protocol for your experiment.
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Faride Noroozi shahri
Raghad Mouhamad I am very grateful for your help and guidance.
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Prinka Goyal
You can use the Dimethyl sulphoxide method for estimation of chlorophyll content. According to me, this is easiest method for estimation of chlorophyll content.
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