I having being maintaining the MB MDA 231 in DMEM, 10%FBS, with antibiotics at 5%Co2, 37C.
I found problem in cells forming the circular fringes as attached in Picture. Might be due to apoptosis or necrosis.
Help me clearing out the deaths cells forming circular fringes ?
These are huge and flattened senescent and/or polyploid cells. It is typical feature for those cells. The culture consist up to 5% of that kind of cells, it is perfectly normal for them.
Thank you Agnieszka Bojko.
But, As this were maintained in 60mm pteri plate, there were around 30+ flattened senescent or polypoid. As captured in image.
How old is your culture? I am throwing away cultures that are in use longer than 2 months (after thawing). They can spontaneously change their phenotype and behaviour, resulting in increased polyploidy and senescence.
I have received the culture from repository which had a passage number of 57. And i am still continuing the culture.
I have discarded the culture..
Thank you Agnieska Bojko for your suggestions.
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