Can anyone please suggest me method for quantification of exosomes after isolation from blood samples?

More Shailender Gugalavath's questions See All

Can anyone please suggest me growth factors required for Human adult dermal fibroblasts?

I have being using the Adv DMEM for now but couldn't maintain the fibroblasts as healthy. I have being observing the slow growth of the cells and blebs, showing some apoptosis nature. So thinking...

17 July 2016 7,087 4 View

How can I identify a genomic DNA sequence from mRNA (Bread wheat)?

I request your kind help, regarding the identification of a genomic sequence from a mRNA sequence. I had identified a mRNA sequence for Ferritin in wheat (EU143671).Now I want to identify the...

24 December 2014 8,118 9 View

How can I perform the computational comparative genomics of Wheat chromosome 1 and known sequences of Aegilops longissima ?

I want to perform the comparative genomics analysis of known sequences of wheat chromosome 1D and all the known sequences of Aegilops kotschyi at NCBI. Can anyone provide me the protocol,or tool...

24 November 2014 4,547 2 View

How to calculate a drug dosage for transdermal drug delivery?

Working on a drug for transdermal drug delivery, I came across the problem of dose calculation. How the dose of API for the transdermal drug delivery is calculated? Is there any role of drug's...

03 June 2014 527 2 View

What should be the In-vitro dissolution profile for bilayer tablets containing different drugs which have different solubility at different pHs?

Recently I've been working on my project of bilayer tablets, in which two layers (one designed as immediate release layer and another one as extended release layer) are to be compressed into one...

21 April 2014 4,535 0 View

What are the associated parameters of mercury pollution in water and soil?

I want to know associated parameters of mercury in urban environment.

17 December 2013 2,778 2 View

How to scientifically prepare a working stock (1ppm) from a stock solution (1000ppm)?

Specifically I want to know about a standard solution (1,2,3,4,5 ppb) preparation of Mercury from Mercury stock solution (1000ppm).

08 October 2013 4,714 10 View

Can someone share a protocol for measuring leaf area index?

Leaf area index for measurement of canopy cover, which is important for me to work on for carry out research.

04 August 2013 9,067 4 View

Why do we use acids for heavy metal extraction?

When we are doing heavy metal extractions from plant samples we use acids like HNO3 or HCL . I want to know what is the importance of these acids in extraction and I need to know about the...

15 May 2013 9,246 23 View

Evaluation of Ph.D. thesis

I am ready to submit the thesis entitled "Study on the effect of MMP-2 gene silencing in the protection of human normal dermal fibroblasts and sensitization of MCF-7 human breast cancer cells from...

01 January 1970 1,938 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Alexander Sasha Vlassov

we routinely use Nanosight LM10 for exosome sizing and count. there are other instrument options- from Nanosight/Malvern, Particle Metrix, iZon. Colorimetric approach might also work, but it will be tricky and not very accurate

Salvatore De Rosa

I recommend nanotracking.

Shailender Gugalavath

Nano tracking requires a instrument?