I have being using the Adv DMEM for now but couldn't maintain the fibroblasts as healthy. I have being observing the slow growth of the cells and blebs, showing some apoptosis nature. So thinking add up some growth factors required to it.
So guys please suggest me Growth factors that can grow Fibroblasts cell healthy.
i have worked with several cell lines of fibroblast and i use just DMEM with 10% FBS (and antibiotic)
they grow beautifully :)
I agree, in my previous lab we have grown dermal fibroblast with MEM/DMEM and 20%FBS and they really grow well. They can be passaged as well as stored for further use.
And i have also seeing the black dot spots in culture ! What might be the reason ?
For black dots probable reason might be your cells are not fine, they might be undergoing apoptosis and you are observing the cell debris. If you find dots in the media or on the cell surface check for mycoplasma contamination.
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