Real Time PCR: What is the justification for acceptable variance within 3 Ct? Is there any publication on this variance?

18 December 2020 1 10K Report

Hi all, I was not able to find any source with evidential justification for why the acceptable variance for internal control should be within 3 Ct and not other numbers of cycle of amplification. In addition, I could not find any publication addressing this aspect too. Anyone could give me a clue on this? I need a publication paper addressing this for my study.

Thank you in advance!

Vipul Batra

Wild Guess: Maybe because a stable housekeeper may be expressed at the 34th ± 3 cycles across all the samples e.g. C and T. Therefore it doesn't matter which cycle rather a stable expression across samples is solicited.

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