How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Does anyone use the ISE electrodes (ammonia, nitrate etc.) to characterise soils samples and plants extracts? What are advantages & disadvanteges?

I will have numerous soil samples/rice rhizosphere sample to characterise as part of the rice microbiome study. I was told that there are ISE electrodes that can be used to measure ammonia,...

19 July 2020 2,066 3 View

Which anti: GRP94 antibody do you recommend?

I am working with C.elegans strain which expresses H.s. GRP94 protein. I am looking for proper antibody to detect GRP94. Do you have some recommendation or suggestions?

13 September 2016 7,214 1 View

Do I need phylogenetic correction to my analyses when I have in data base 11 species?

Dear all, I am performing different analyses (ANOVA, ANCOVA, correlations etc.) and I am not sure if I need phylogenetic correction. On my dataset I have 11 closely related species from Carabidae...

22 November 2015 5,406 12 View

I'm looking for cell dye, which changes it's colour when entering the cell. Can anyone help?

18 October 2015 6,368 3 View

Does anyone have experience with polytypes of CdI2 and their structural calculations?

Or maybe know someone who has experience in this field?

08 February 2015 4,806 3 View

How can I analyse fluorescent images of Double/triple positive cells?

Hi I am using a Columbus for fluo images analysis. I need to define double or triple positive cells. My nuclei are stained with Hoestch (all cells), Sytox (dead cells only) and FITCs (apoptotic...

05 January 2015 3,830 4 View

Does anyone know how to soften the eyeball for histology (paraffin technique)?

02 December 2014 7,688 3 View

Can anyone help me to improve my Lipase assay Kwon and Rhee?

Could anyone help me to improve my lipase assay. I need to study the lipase activity produced by Bacillus saliphilus isolated from alkaline waste lime. I try to apply the method by Kwon and Rhee....

16 September 2014 9,717 29 View

Does anyone know about an ELISA kit that would measure small amounts of human urocortin 2 (UCN2) in serum/plasma?

Detection range around 10-20pg/ml. I have used one but it didn't even detect UCN2 added to the wells. Thanks.

20 May 2014 6,892 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View