Rationale behind selection of 3T3-L1 adipocytes & C2C12 Skeletal muscle cell lines?
On 27 May 2024, accidentally I removed 25 papers from my list of publications on Rsearch Gate. How to restore deleted papers?
28 May 2024 3,927 1 View
need answer in a tleast 20 lines
21 April 2024 5,489 0 View
I treated CNTs in reduced Nickel chloride solution using sodium boro hydride. After centrifuing i got mixture of CNT and nickel. how to seperate CNT? i have done this for decorating CNTs wit...
20 April 2024 6,574 1 View
I have AFM scans of a uniformly textured surface of 16x16um size. I would like to obtain the slope distribution of the surface using Nanoscope, as I plan on trying to relate this parameter to the...
10 April 2024 5,024 2 View
Over the last 10 years, I have been using the Mendeley citation manager. Due to their new policy, it is not working as smoothly with MS Word. Therefore, I am looking for a good alternative.
10 January 2024 3,460 5 View
In CRISPR/Cas, we design guide to make double strand break in a specific site of the genomic DNA. I am curious to know, whether the gRNA-Cas9 complex has any possibility to bind to the messenger...
25 December 2023 630 1 View
using solution based method
17 November 2023 8,243 1 View
Iam Dr M HARIKRISHNA doing research in organic chemistry, I need help for docking studies?
03 November 2023 9,336 3 View
I want to measure the work function of fused silica. Is it possible to measure the work function using UPS? I have seen several papers using UPS, but did not get the concept behind that. Does...
02 November 2023 3,779 4 View
how can we find out the volume of chamber 2, using the equation state of an ideal gas by isothermal expansion of a known volume of a gas from chamber 1 to chamber 2
01 November 2023 7,811 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...
03 August 2024 835 3 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View