How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Why green band returns more inliers than NIR band during image registration?

Hello I'm using image registration over the bands of the micasense red-edge camera. Using RedEdge band as the fixed image. After removing inliers I can notice that inliers of the NIR band are...

13 January 2021 3,980 2 View

How to model the causal effect of working part-time compared to full-time on health?

Hi there, I have to estimate the causal effect of part-time work compared to full-time work on health. I have an unbalanced panel data set with t=2008-2017, except for year 2009. The main issue...

11 December 2020 3,170 3 View

How to correlate bacteria community data with volatile profiles and with fly community data using ordination techniques?

Carrion Ecology: What is the best way the statistically analyse the correlation of bacteria community data to volatile profiles and to fly community data across 3 time points using ordination...

24 November 2020 5,831 3 View

Does anyone have any advice for Intracellular Staining of methyltransferase for flow cytometry?

Hi All, I have been attempting intracellular staining of a methyltransferase and have been running into issues with consistency. I have attempted sequential staining with a PE secondary and have...

20 November 2020 2,314 1 View

Problem executing MrBayes. Help?

Hi, I'm trying to run MrBayes 3.2.7 with a matrix of 37 species and 20561 nucleotides. I consistently receive the following error: MrBayes > Execute...

18 November 2020 3,867 3 View

Which commercial DNAse do you recommend me?

Which is the best DNAse (or DNAse kit) you have worked with? I am looking for a good DNAse that can remove DNA from an RNA sample in one pass. Material would be used for future RT-qPCR analysis....

18 November 2020 8,769 3 View

Is there same kind of limit when annealing two DNA products?

Hi there I'm planning on annealing two PCR products to perform some mutagenesis in a plasmid. I was recommended to try and keep it to 600pb each, for example, but for one of the plasmids there...

15 November 2020 8,656 3 View

How many casual deaths constitute scientific evidence? Do we know the risks of repeating influenza vaccination?

Italy (2014). Around 35 death after and very closer to the flu vaccination Spain (2020). At least 45 death reported in correlation with adyuvate flu vaccination and 6 time risk to died with...

22 October 2020 2,746 2 View

What is the relationship between plant hormones and plant allometry?

Hi, i'm looking for bibliographic references that explains the relationship between plant hormones and plant allometry (relation between the size of an organism and aspects of its physiology and...

21 September 2020 9,653 6 View

Does anyone have kinetic and kinematic dataset for gait classification?

I want to differentiate between normal and abnormal movement. For this reason, I am looking forward to this type of dataset.

01 March 2021 9,485 1 View

Anyone give me assistance with analysing Gait Data?

I currently have collected Gyroscope/Accelerometer data (X/Y/Z) for my dissertation, but not very knowledgeable on how to process my data and apply Machine Learning? Can anybody help?!

24 February 2021 9,610 3 View

Why does the accuracy of NN decreases when two datasets are combined?

I am working on gait analysis using a feed-forward NN. When I use data from the accelerometer (3 columns for each axis if stored in a data frame), I get an accuracy of 95%, and when I used data...

07 February 2021 9,654 6 View

Is there any research on universal gait and O2 consumption patterns in recreational runners?

15 January 2021 6,479 1 View

Is it rational to identify differentially expressed genes from tissues (in silico) and examine gene expression on the associated cell lines (wet lab)?

I am going to evaluate the gene expression levels in the MKN45 cell line (gastric cancer). Before that, I want to identify critical differentially expressed genes obtained from tissues available...

20 December 2020 3,774 6 View

Is all normalization for microarray the same?

What i know, microarray data has vsn, loess and quantile normalization . i am dealing with GEO microarray datasets where i have to find the differential expresssed genes for NPC. im planning on...

10 December 2020 1,496 3 View

Which automatic data processing for angle detection in Plugin Gait (VICON) do you use?

I would like to analyze gait cycles (running) - is there a smart way for cycle detection and calculation of the angles for each joint (average/maximum) for a certain amount of cycles - without...

07 December 2020 2,586 2 View

Does anyone know of cases of complete eye evulsion (eyes jumping out of their sockets) in ancient or medieval sources?

01 November 2020 1,234 3 View

Can anyone please provide information in regards to the utilization of the exoskeleton as a perturbative tool for gait/balance therapy?

There exists a considerable body of literature on the application of exoskeleton as an assistive tool in the area of gait or balance therapy. I am interested in finding out more about the...

06 October 2020 9,317 3 View

How does forest effect the water cycle at basin scale?

Though the effect of trees or forests on water is well known, is the effect considerable when it comes to larger scale, basically at basin level? Taking the example of Ganges basin, Do you think...

20 September 2020 7,260 7 View