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Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

The role of binocular diplopia in the dental treatment of visual field defects?

In dentistry, we sometimes need ‘binocular diplopia’ when dealing with complex dental problems, which means using different angles and tools to ensure the treatment is effective. In your opinion,...

09 July 2024 3,898 0 View

Quelles perceptions et quelle place pour la langue chinoise en Afrique ?

Lorsque nous nous posons cette question , nous nous basons sur un fait et constat très simple : Dans les manuels d’apprentissage du chinois, qui tiennent lieu de programme pour l’instant dans les...

08 July 2024 7,650 3 View

Why Q5 DNA Polymerase failed in PCR short primers?

Hi everyone! I am using a Q5 high fidelity DNA polymerase to amplify a 6.7 kb plasmid. The PCR with Q5 went well with longer primers (~55 bases) at 70-72 degree C. However, I keep failing in the...

17 June 2024 1,734 4 View

How can advances in technology, such as barcoding systems, improve specimen management processes in diagnostic microbiology?

The question is based on the article “Tenets of Specimen Management in Diagnostic Microbiology” by Rajeshwar and Pathak. It explores how advances in technology, specifically barcoding systems, can...

10 April 2024 9,863 1 View

How to add an alternative name?

Hi! I got divorced so I changed back to my maiden name. How to add to my profile? It’s now Grace van Leeuwen/ Grace Caroline van Leeuwen

26 February 2024 3,482 1 View

Lifting Mesenchymal stem cells with trypsin proving difficult?

I am in the process of learning how to culture MSCs that I have flushed from the bone marrow of mice femurs. So far, the process runs smoothly until I attempt to trypsinize the cells, I attempt to...

21 February 2024 9,211 0 View

What are the factors that are needed to be considered in choosing the right antibiotics that can help physician in using antibiogram?

the concept is derived from Sundareshan & Khardori (2019) regarding the usage of antibiogram as Public Health Tools to Slow Down Antibiotic Resistance. it is indicated in the article that...

06 February 2024 2,293 1 View

Competition Assay of peptides in flow cytometry?

Hi, I performed a competition assay of serially diluted concentrations of the unlabeled peptide by adding a constant concentration of the FITC-labeled peptide. I have observed higher fluorescence...

06 January 2024 6,949 6 View

Only Irish reference on autism on ID people in regarding to respect and dignity?

. i need articles that can help me find this information

08 December 2023 5,734 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

How do I run a dead well test for a QuantStudio 5 qPCR machine?

I am preparing to run a lot of samples on a 384 well plate and I wanted to test if any well in my PCR machine is dead so as not to lose any data. I would really appreciate help with how I can do...

24 July 2024 8,172 1 View