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What will be the appropriate data collection method and data analysis that I can employ with may experimental research on squash germination?

I am conducting an experimental research on the germination enhancement of squash affected by mechanical, chemical and thermal scarification methods..i have researched about time- to-event method...

27 December 2020 7,133 3 View

How to conduct and interpret meta regression using prevalence rates in Stata?

I have conducted a meta-analysis of prevalence rates using metaXL. There was a large amount of heterogeneity in the studies so I now need to look at (categorical) moderating variables. I have...

15 October 2020 4,630 4 View

Are there available scales or questionnaires to measure change management?

Seeking to connect with authors who may want to share their scales or questionnaires to measure change management.

27 September 2020 374 6 View

Early amplification in both NTC and NEC in qPCR (using taqman probes)?

Hi! I've been trying to decipher what happened to some of our runs with early amplification in the NTC and NEC. This has happened to us twice now. For background, we are using a mastermix of PCR...

09 September 2020 4,559 5 View

Help with meta analysis using prevalence rates?

I have conducted a systematic review into the prevalence of victimisation in individuals with Autism. This provided a large number of relevant, high quality studies which I have synthesised into a...

14 May 2020 5,398 3 View

Why should a Journal that is Social Science Citation Indexed in the Web of Science not be listed on the Journal Citation Report (JCR)?

A Journal that is in the Web of Science (WoS) as social science citation indexd but it is not found on the list of Journal Citation Report (JCR). The website of the Journal shows also that it is...

04 May 2020 6,730 5 View

Which Augemented Reality (AR) authoring tool is easiest to use to develop an AR app by someone who does not have training in Computer Science?

I would like to introduce Augmented Reality (AR) as one of the ways to learn anatomy to our students, and I have been looking at some literature in this regard. I noted from several articles that...

15 April 2020 3,990 5 View

How to identify hypothetical proteins in bacterial genome?

I have used BLASTp and InterPro to identify the proteins in a new species of bacteriophage, which has given putative identification to roughly half. What are other ways I can identify more of the...

24 March 2020 6,706 3 View

P. aeruginosa growing with various colours?

My overnight cultures of P. aeruginosa PAO1 can range from a creamy yellow colour to a bright green - what could be causing the different pigment production? I always prepare the cultures the...

02 February 2020 7,246 3 View

Can anyone suggest me any website or source to find out green consumer /sustainable consumer data/index?

green consumer /sustainable consumer data/index?

24 January 2020 810 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View