I am following the protocol from:
https://academic.oup.com/nar/article/43/22/e152/1805371 - heading "Assessment of the enrichment"
However, the protocol sample is Lambda DNA, whereas I am using human DNA. I wish to quantify withing qPCR both the targeted DNA and non-targeted DNA from the capture enrichment. Designing primers for the targeted region, with is an LINE-1 element, is fine. However, how would one design primers for the remaining genome to assess the non-targeted capture? 1 or more house-keeping genes? If so, could someone point me in the direction of a suitable paper describing this.
The sample is human blood.
Thanks in advance for suggestions.