Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Can a random-intercept cross lagged panel model (RI-CLPM) be indentified with just two measurement points?

I am currently planning out my masters' thesis design and will be working with a large data set with variables that have been meseasured at two time points. Intially I thought of just using a...

21 January 2021 9,384 4 View

Why are species accumulation curves shorter in R than Primer?

I have previously used Primer to output species accumulation curve results (including Chao, Jacknife and Bootstrap estimators), and from memory if I had 40 study sites, there would be 40 numbers...

23 December 2020 8,514 2 View

How to calculate sample size for 3 way Mixed Between Within Subjects ANOVA?

Hi all, I have been trying to figure out a way to calculate a priori sample size for a mixed anova. I have a study that uses 2 x 7 x 2. The last IV is within subjects. I appreciate any help....

23 September 2020 3,635 3 View

What opinions do you have on plate readers you've used?

Hello everyone, Our lab is currently looking to purchase a plate reader and I was interested in seeing what opinions people have about some that they have used. Some of the other labs on...

07 September 2020 2,736 3 View

How to do metaphase spread IF staining?

Pls help! I would like to do IF staining with 2 antibodies on a metaphase spread. But I dont have a cytospin! How to do it without cytospin? And im using methanol:acetic acid fixation.

16 August 2020 488 1 View

Is it possible to remove pollen from museum bee specimens?

I have recently extracted genomic DNA from some museum bees for sequencing, and from first look there appears to be some pollen DNA in the extract. I've had a Google but can't find anything...

25 May 2020 8,423 2 View

What is the best way to probe for Pyk2 activation?

I have been trying to look at Pyk2 phosphorylation in my cells (human monocyte derived macrophages) for about a year via Western Blot, and am having a massive amount of difficulty doing so. I have...

16 April 2020 6,662 0 View

What effect size calculation to use for meta-analysis?

I am conducting a meta-analysis for the association between TNBC and BRCA1 and BRCA2. Can anybody help me as to what 2x2 frequency table to use- standardised mean difference, correlation...

05 March 2020 4,992 3 View

What is a good scale to assess prejudice and approval for stay-at-home fathers?

Hello, I am psychology student at Eastern Oregon University. I am currently working on a study that assesses prejudices and approval for stay-at-home fathers. I have not found a good scale to...

25 February 2020 4,465 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Is there any rich, publicly available English literary corpus?

Hi everyone. I am looking for a rich corpus in English literature, a one which includes literary prose ( e.g., novels or short stories) . can anyone propose a good one? Best regards Fatemeh

01 March 2021 1,970 2 View

Is there any teachers based in UK who would like to take part in my study?

24 February 2021 4,833 3 View

What low-cut filter (high Pass frequency) to be used for response spectrum (Tripartite plots) ?

What low-cut filter (high Pass frequency) to be used for the response spectrum (Tripartite plots)? I am currently working on differentiating between hard/soft site responses in Indo-Gangetic...

21 February 2021 9,273 2 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

Which database is the most suitable for checking English bigrams frequency?

Which database is the most suitable for checking English bigrams frequency? We want to assess the frequency of different English graphemes. Most of the databases that we have checked seem to...

18 February 2021 4,565 3 View

Change of First name?

In some publications you refer Deivid Chica (with Barranco) but the true first name is Maria Dolores Chica , in spanish Ma. Dolores Chica. Before I answer yes is an author Deivid Chica, but...

18 February 2021 5,004 3 View

How can I use Permanova to analyze individual variation with RNA seq transcripts and multiway Anova design?

I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...

13 February 2021 7,217 4 View

How reliable is estimation of copy numbers by absolute qPCR?

Hello, I would like to compare the expression levels of several genes in insects, so I prepared standard curves and estimated copy numbers of each sequence in my samples. But for one of the genes,...

11 February 2021 8,421 13 View

Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View