How to determine the population and sample size?

15 February 2021 9,340 4 View

What is the most suitable program for determine the state of synergesm, antagonism or addition of mixing two or more toxicants?

In a toxicology experiment the mixing pattern of two or more toxicants required for the physiological or lethal effect. what is the suitable program or equation that I can use?

06 January 2021 7,419 3 View

How can i measure traces of Hg2+ spectrophotometrecally, ?

I want to measure a low concentration of Hg2+ in a solution spectrophotometrically, ia there an indicator or something i can use as an indicator for Hg2+ to color it and make it easy to measure

14 September 2020 3,575 3 View

A question about Conquest or WebCSD search in-house databases?

Dear all I want to ask help in searching about all crystal structures that contain : Zn/W/P or Zn/W/Si Can anybody help me in case of having access to conquest or WebCSD and his/her help will be...

25 August 2020 1,883 3 View

What are the light quality,quantity and intensity requirements for growing mango under greenhouse or poly houses ?

What are the light quality,quantity and intensity requirements for growing mango under greenhouse or poly houses ?

17 August 2020 2,823 1 View

Find location (x,y) where expression is satisfied in COMSOL ?

for 1-D axisymmetric model. I need to find the coordinate r where the variable x is equal to x0 for all times? find(r, x==x0) .... What a COMSOL operator should I use for that?

02 July 2020 5,098 8 View

A question about Mendely bibliography ?

Dear all, My question is how to do the attached citation bibliography using Mendeley? i.e. merging many references in one number and letter of each.

28 June 2020 5,084 2 View

Expanding mouse T cells in serum-free medium?

Dear All, I would like to expand T cells in serum-free medium then inject without wash in mice? Is there is a special medium for that purpose? Thank you. Best, Hossam.

12 June 2020 6,112 4 View

Can pilot study done under the same sampling frame of parent research?

12 April 2020 5,110 3 View

What is the main difference between optimizing an airfoil using artificial neural network (ANN) and convolutional neural network (CNN) ?

Hello, I wonder what is the difference between optimizing an airfoil using artificial neural network (ANN) and convolutional neural network (CNN) ? How much do the output differ using each method...

17 January 2020 6,719 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

Found that there was a multiple band when amplifiying targeted gene ?

i few days ago amplified specific gene using PCR with specific primers, but there was a multiple band in electrophoregram i found. out of 10 sample amplified, there was multiple band and there was...

18 February 2021 5,131 5 View

Why is there no protein expression but antibiotic resistance in transduced A549 cells with pLVX-TetOne-Puro lentivirus?

Hi, I have been trying to transduce A549 and Calu-3 cells using the Lenti-X Tet-One inducible expression system (TakaraBio). My gene of interest is inserted in a pLVX-TetOne-Puro plasmid and has...

15 February 2021 8,462 3 View