Hossam Murad

5 Questions 8 Answers 0 Followers

Questions related from Hossam Murad

Cloning vector for high GC ratio gene? - is GC ratio a criteria in choosing vector?

I'm trying to clone a ~900 bp gene which has a high GC ratio (about 75%) in E. coli. (Top10). The thing is, I'm not sure if the ratio of GC is a criteria in choosing the vector or rather, if...

17 May 2016 429 6 View

Cloning vector for high GC ratio gene? - is GC ratio a criteria in choosing vector??

I'm trying to express a my target gene which has a high GC ratio about 75% in E. coli. The thing is, I'm not sure if the ratio of GC is a criteria in choosing the vector or rather, if there are...

17 May 2016 5,043 1 View

Why is there an up-regulation on DNMT1, DNMT3a,DNMT3b gene expression after gamma irradiation?

I treated an Astrocytoma cell line to 10 gry of (gamma Irradiation), I noticed that an up-regulation of DNMT1, DNMT3a and DNMT3b gene expression. is there any explanation?

03 August 2015 1,118 4 View

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22 May 2013 7,218 1 View

How can I detect the membrane receptor of MDA-MB-231 for my extract?

I have a red seaweed extract, so I want to know which membrane receptor binds to my extract to activate the pathway?

01 May 2013 6,000 3 View