Purple macckonecky agar results interpretation?

More Saleha Infal's questions See All

How to solve g_mmpbsa error?

Program: g_mmpbsa, version 2024.1 Source file: extrn_apbs.cxx (line 152) Fatal error: Failed to execute command: $APBS pybYcUWA.in --output-file=pybYcUW.out

07 August 2024 6,066 0 View

Why binding energy is in positive number after performing mmpbsa?

I performed mmpbsa of protein-protein complex simulation on gromacs. My binding free energy is +166781. This complex is stable when experimentally produced in wet lab. In index.ndx I grouped...

19 July 2024 4,120 5 View

65% saturation of ammonium sulfate in 100 ml? (for protein precipitation)?

Hi All: I need to precipitate extracellular protein from supernatant. A bit confused with ammonium sulfate calculation. Should I be adding: 1. 65g of ammonium sulfate in 100 ml of...

09 July 2024 5,508 0 View

How to find the expected Kd value of a recombinant antibody?

We produced scFv of an antibody from bacteria. Now we want to establish dissociation Kd of antibody using quantitative elisa. How can i know the expected Kd value (range in nM, or mM)?

04 June 2024 2,602 5 View

Are protein models designed computationally patentable?

If we design proteins like tetrabody or triabody with unique binding sites in silico without further wet lab validation can we patent that structure and procedure?

01 June 2024 2,493 1 View

Opinions required on determination of function of hypothetical small protein?

So, I am working on a small protein (X) of Haloferax volcanii. It shows these results: 1. Mutant strain grows poorlycompared to wild type strain under low iron condition. 2. Growth of Mutant...

14 May 2024 9,037 4 View

How to start GROMACS simulation from a specific checkpoint?

My simulation suddenly stopped due to some reason. Now i cannot restart the simulation from previous checkpoint because it was not written properly. my _prev.cpt file is also corrupted....

18 April 2024 2,461 0 View

Suggest bioinformatics journals?

Please suggest bioinformatics journals which do not need wet lab experiments.

10 March 2024 2,919 6 View

How to simulate a complex at various pH on Gromacs?

We want to simulate a protein-cabohydrate complex at various pH ranging from 6.8 to 7.4. Can we do this on Gromacs?. Do we have to simulate complex at different constant pH or the system will...

05 March 2024 1,633 3 View

What protonation state will Histidine have at pH 7.4?

Histidine can adopt 3 protonation states: HIE, HID and HIP. Which protonation state will it adopt at ph 7.4

20 February 2024 8,503 0 View

Is skin yellowness an numerical or ordinal variable?

I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...

11 August 2024 4,793 1 View

Using OBD technique i am trying to measure laser induced shockwaves velocity i found that at start velocity increases and then decay?

i am unable to interpret why its increases in start as shown in figure

11 August 2024 2,179 1 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

How can I interpret the data without the need of solving it manually?

How can I interpret the data gathered without solving?

03 August 2024 9,054 3 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

Roger Bayston

I think you mean MacConkey agar

Saleha Infal

Yes @roger bayston

Michael J. Benedik

Traditional MacConkey agar has bile salts which are there to inhibit the growth of many non-enteric bacteria. The color indicator will depend upon what sugar is used in the medium. Again traditionally it has been lactose (since E. coli is a lac fermentor) but you can use other sugars as well. Lac+ would be bright pink and Lac- would be more yellow. But again it would depend on which fermentable sugar you added.