Ok. But this is analytical scale, exept when they have tool for syringe (1ml). That will be good. I thinking about some kind of spe...(?), mean cache Everything and elute only aminoacids. Is one paper about this but I don't have sure :)... I checked...

can You Krzysztof send me some simple guide for SPE approach? I have C18 adsorbent in bulk....

thanks Krzysztof, i try tommarow with this proposition...

Would the amino acids come out with the c18 or mix with the methanol on beads?

#1 subject from Sarah Oertly:

C18 its a long chain (18th atoms of carbon with adeuqate -CH3 groups on each carbon) STABLE connect on the surface silica gel SiO2, you can elute only sample and solvents and other external chemicals (for example: derivatization, etc...) ... and in some few casses small part of active groups like C18 in non-comercial adsorbent, but this is not problem... (XAD-2 working like that)....

#2 I think - this method which Krzysztof B. suggested - its for fractionation of amino acids (from acids to basics way)... i thinkg...... :-/ - C18 adsorbent have this patch way of elutions... in HPLC with OPA and FMOC its almost this same.

#3 but its could be very good methods for separations on each types (AA), but its will be more helpful when we create methods for general separations, mean only AA from (i hope) dipeptides (exept cystine - beacouse in some cases its is a marker for cysteine) PROBLEM 1.

If someone wants make some team work in this subject its will be very exciting. I have HPLC - DAD and some experience in aminoacids analysis, it is possible to apply GC-MS or GCxGC-TOFMS, for this. It is simple way with derivatization of

AA.

my email:

[email protected]

ok then, what kind of preliminary study Y you have Krzysztof in your mind - about optimize of hydrolysis?

You can see AOAC method