I need some journal articles that examined the associations between CMV being positive or negative and different features that are associated with CMV like weakness and slowness.
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
I'm working on the atmospheric water harvesting, and i synthesized MOF by adding some materials into precursor and changing the conditions. (i followed Hydrothermal method) Actually, if MOF is...
24 July 2024 1,515 0 View
UK's Well-online Science Press sent an invitation to be a co-author and offers a payment of $30 per review.
22 July 2024 1,695 8 View
One of the challenges in ensuring quality and integrity in blended learning modules is cheating especially when it comes to assessment (Tests, exams, and assignments). Now one of the solutions...
21 July 2024 6,741 0 View
I want to express STK4, and I've searched the paper for reference. When I check the protein kinase domain sequence for that kinase on Uniprot, it's 30-281, but the paper expresses the protein...
20 July 2024 4,951 1 View
Photo-electric effect A light particle moving towards a surface cannot (by collision) force an electron particle to move away from that surface. A light wave can instead make interference with a...
19 July 2024 8,389 9 View
I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...
16 July 2024 7,475 1 View
Hello everyone, This soudns like a strange question, but I created five XTC files using five different versions of GROMACS some years ago. All other files are missing, and I want to repeat the...
16 July 2024 7,205 2 View
I am preparing cancer cell conditioned media to study the effects of substances secreted by cancer cells on other cells. In some papers, conditioned media is prepared using FBS-containing media...
11 July 2024 7,063 1 View
Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...
11 July 2024 4,812 5 View
Hello, everyone. I would like ask one question. I transfected fibroblasts with EF1A-myod-puro using lipofectamine 3000. Promoter for puro is CMV. After transfection 48 hours, I did puromycin...
23 June 2024 4,902 3 View
Hi~ We are making different Cas9 plasmids such as regular spCas9, base editing and prime editing under different promoter such as CMV and CAG. During selection of colonies, we always get a good...
22 May 2024 7,336 0 View
I need to express some peptides in cell-free system (fe TnT) but my plasmid has CMV promoter (no T7 or Sp6).
21 May 2024 4,964 4 View
Hello all. I grew some cells for virus infection. I infected the cells with CMV (Cytomegalovirus) 8 days ago. The cells have not started lysis yet, but the plate is about to get dry. I wonder if I...
08 May 2024 7,052 3 View
I have plant sample with mixed infection of CMV and potyvirus, is there any way I can separate CMV only?
10 January 2024 3,972 1 View
I have cloned my genes in a CMV vector and transfected them in HEK293 cells. After harvesting the cells, the expression level at the transcriptional level is increased compared to the control...
23 December 2023 2,507 10 View
I am having a doubt regarding CMV promoter and SV40 promoter in mammalian expression vector 1. Why CMV and SV40 promoters are used in mammalian expression vectors why not others? 2. There is a CMV...
05 December 2023 1,830 3 View
Hi I was wondering whether CMV and EF-1alpha promoters can be put in a single plasmid? Are there any compatibility issues between the two promoters? Thank you.
31 October 2023 9,919 2 View
In accordance with the research titled "Transfusion Services in Tropical Africa: Challenges and Prospects from the Nigerian Perspective" conducted by Sagir Gumel Ahmed in 2022, unlike other...
06 September 2023 4,740 1 View
I used 0.5 ug plasmid. Cell confluency around 60%. Transfection time is 48 hour. I used empty plasmid (pCMV-3Tag-4A) and only Lipofectamine as a negative control. However, I used EGFP plasmid tag...
03 August 2023 1,883 2 View