PRP for wound healing?

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How to open an old ".MTS" file containing spectroscopic data?

Hi supreme hive-mind of the internet, we have some old *.MTS files that are supposed to contain spectroscopic data, probably some coordinates and a long list of single line spectra. Basically,...

16 February 2021 9,385 2 View

How to compute the distance in km between two points inside the Earth?

Hello everyone. I am looking for a simple formula to compute the distance in Km between two points inside the Earth. I do not mean the usual problem between to cities (distance along the surface)...

02 February 2021 3,455 4 View

How can I quantify background noise in a bioacoustic file?

I am using Audacity to analyse the presence of birds and frogs recorded with a SM4 recorder (Wildlife Acoustic). Background noise (rainfall, wind) is an important variable to consider that can...

28 January 2021 2,989 3 View

Does anyone know how to get a scopus or sciencedirect report of the trends in recent years on a specific research topic?

I am trying to obtain a trend report on a specific issue at your instigation in recent years

28 December 2020 848 2 View

Do you think there are any prospects for hydrogen as an alternative to natural gas when operating a gas turbine?

At the moment it is very expensive, the price of 1 kg of hydrogen is from $ 2-5. Are there any prospects for green hydrogen? or will we extract hydrogen from gas? what promising technologies can...

19 October 2020 1,954 7 View

Containment level laboratory 1 or 2?

We are renting a small laboratory (135 sqft) where we mainly do chemistry. We will receive from overseas samples of 1 kg of Purple non-sulfur bacteria which has been fixed and disinfected with...

19 March 2020 6,757 4 View

How can I project 3D objects on 2D images??

Hello! From 3D images stacks I have recovered some objects. Now I need to project those objects on 2D for further analysis. Any idea, any software, plugin, etc?

21 January 2020 4,144 4 View

Does anybody knows if there is a metodology of acute ethanol toxicity for neuroprotection studies that uses functional foods?

I recently read an article regarding the use of melatonin as a neuroprotective agent using acute ethanol exposure for 1 day and it had good results, I want to know if exists an article using...

03 January 2020 9,709 2 View

Color reference chart for microscope?

Hello, Any recommendations on color reference charts for an stereoscope? Thanks!

09 October 2019 8,422 2 View

Is there a way to normalize my western blot bands since i loaded equal volume of seminal plasma (not based on protein concentration)?

I loaded 10ul of raw seminal plasma sample + 10 ul Bolt LDS buffer +4ul Bolt reducing reagent. I did the transfer using iBlot2 dry transfer system and used One Pierce ECL protein detection kit....

06 May 2019 6,912 3 View

Which alginate is best for making gel for wound dressing application?

We are looking into utilizing alginate as a gel coating on an HDPE substrate for drug delivery. I would like to ask the community about their experience with alginate and which commercial sources...

15 February 2021 4,319 4 View

Endothelial Functional Assays & Conditions?

Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...

05 February 2021 7,276 1 View

What questions should i include in my questionnaire?

I am writing my dissertation, about adults who trained at elite status in their youth, i am constructing a questionnaire with the goal of seeing, how many sustained injury, how many sustained...

28 January 2021 4,787 3 View

How do i analyze scratch assay data obtained thru imageJ?

hello. i manually measured the area in each image. the output i got was area/mean/min/max then i put all the data into excel, and also added the following columns: group names/date/hour pic was...

19 January 2021 3,936 3 View

How does a cell repair itself? Can you relate this to mathematical genetic algorithm?

I am on a quest to solve how a cell repairs itself through encoding-decoding of proteins. Is there any link to genetic algorithms to solve age old questions such as aging and how we heal?

14 January 2021 9,958 1 View

What's the reasonable loading control of nuclear protein in acute injury?

What's the reasonable loading control of nuclear protein in acute injury? Is HDAC1 or Histone H3 a resonable loading control of nuclear protein in acute injury model? How to explain total my total...

06 January 2021 1,607 2 View

Will the serum of healed patients can be considered to make a vaccine/drug against COVID-19?

22 December 2020 2,180 4 View

What are the morphological changes of PC3 cells during autophagy and/or apoptosis?

I was performing wound healing assay seeding 300,000cells/well in 6 well plates with 2mL RPMI medium. At 0hr after intervention, the cells looked like the first picture. At 24hr after...

18 November 2020 9,671 2 View

Is there some specific drug to induce injury only in muscle fibers without affecting peripheral nerves?

I would like to induce injury in skeletal muscle fibers to study muscle regeneration but I would like to preserve nerve terminals intact. Could it be possible? Is there some specific drug? Thank...

30 October 2020 9,077 4 View

Why measuring drug release rate in vitro in a buffer?

I notice in many articles, researchers measure the loaded drug (e.g. in wound dressing) releasing rate in a buffer (e.g. acetate buffer). Why should we do like this? Drug releasing rate in a...

21 October 2020 7,152 1 View