Hi. I'm doing patch clamp experiments in cultured DRG neurons and I would like to investigate the expression profile for 10 - 20 different membrane proteins known to be expressed differentially in sub-populations of my cells. It would be ideal if I can get a single cell semi-quantitative or quantitative result. Is there a consensus protocol for doing this? or a 'most-used' one?. I will thank any reply pointing me to a useful reading. Thanks & regards
This paper may provide a good protocol:
http://www.biomedcentral.com/1471-2199/11/82
Additionally, I recently reported a very simple method for isolating single cells compatible with downstream RT-qPCR which may be helpful to you. To adapt the pipeline you would isolate cells first, then patch clamp, then continue to the lysis stage in the protocol. The paper is referenced below.
Good luck!
Article A convenient, optimized pipeline for isolation, fluorescence...
This paper may provide a good protocol:
http://www.biomedcentral.com/1471-2199/11/82
Additionally, I recently reported a very simple method for isolating single cells compatible with downstream RT-qPCR which may be helpful to you. To adapt the pipeline you would isolate cells first, then patch clamp, then continue to the lysis stage in the protocol. The paper is referenced below.
Good luck!
Article A convenient, optimized pipeline for isolation, fluorescence...
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