How to calculate age-standardized prevalence ratio with 95%CI?

I need to calculate the prevalence ratios to show the trend of prevalence of a drug group used by pregnant women over the years (2001-2018). I would like to use the year 2001 with age distribution...

05 January 2021 7,986 5 View

Does CD31 indicate cell viability?

Hi, I am wondering if the immunofluorescent staining of CD31 antibodies can both mark endothelial cells and indicating their viability. In other words, if I perform immunofluorescent staining for...

23 December 2020 5,815 4 View

Analysis and implementation of Kalman filter in fixed-point arithmetic?

I would like to implement a Kalman filter for tracking applications for IoT nodes. I cannot do floating point on our nodes but only fixed point. Could you recommend some good references?

23 December 2020 4,813 3 View

How to increase titer of Herpesvirus, Adenovirus?

I'm working with Herpesvirus and Adenovirus. For Herpesvirus, I use Vero cell line to activate and check virus titer. For Adenovirus, I use Hela cell line. Recently, I could activate them, but...

16 December 2020 4,073 5 View

How big can a enhancer deletion be ? how can we obtain homologous deletion clone ?

Hi all, I have tried to use Crispr Cas9 ( plasmid px333_cas9_mCherry) to delete some enhancers. I designed sgRNA using Chopchop website to target both sides of an enhancer to induce homologous...

13 December 2020 942 3 View

ELISA detection cytokine in human serum (common dilution fold)?

Hi everyone, I'm gonna try to detect the IL-10, IFN-y, TGF-beta1 level in the human serum by ELISA. I bought the ELISA set from the BD contain Capture Ab, Detection Ab, Enzyme reagent, Standards....

09 December 2020 3,369 5 View

How to calculate the fractional vegetation cover (FVC) from Landsat imagery?

Hi folks, I wonder if anyone know how to calculate the fractional vegetation cover (FVC) from Landsat imagery? As far as I have read about that NDVI, NDVIs, and NDVIv are three required input...

07 December 2020 4,054 2 View

What is the main difference of diluting HCL in methanol as opposed to water?

I am hoping to determine the mass per unit area (as per DIN 50938) of a black oxide coating. I use water to achieve the required 1mol/l hydrochloric acidic solution, but a fellow lab had used...

06 December 2020 4,806 1 View

Is it possible to identify/classify rubber using satellite imagery with machine learning?

As of now, I am trying to explore the potential of using high/very high-resolution satellite imagery with machine learning (ML) to identify or map forest cover (e.g. rubber). As a newbie in this...

17 November 2020 7,187 3 View

Cloudy brain samples in sucrose solution?

I have some samples I left in sucrose for a couple of weeks after perfusion and was wondering if these samples are still salvageable? I perfused the brain, then they were fixed in 4% pfa for 24...

15 November 2020 1,728 2 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View

Hi, I would like to test different disinfectants and its effects on biofilms in microplates, which paper has the best protocols!?

I need to do it only in microplates and read the OD value for the results. I cannot use any other assays except microplate. Please help me with this

07 December 2020 5,698 3 View

Biofilm Biofertilizers- New Generation biofertilizers?

Biofilm Biofertilizers-It is being applied to adhere the surface of soil and plant matrix containing consortia of various micro-organism that cut down chemical fertilizer. It is cost effective...

12 October 2020 4,883 13 View

When gowing biofilms in vitro, why is the media changed following the initial attachment?

Hi all, I've seen in a number of publications involving the growth of biofilms in microtitre plates, that the media is changed following an initial attachment phase (which can range from 2 - 8...

01 September 2020 7,004 3 View

Can bacteria and biofilm on a filter be seen with SEM without a dehydration/fixation step?

I have a filter with some bacteria and other particles that is going to be analyzed with SEM. I am wondering if anyone knows if you skip the glutaraldehyde/ethanol/critical point drying steps if...

01 September 2020 4,929 4 View

Why do I keep getting a biofilm instead of single colonies?

16 April 2020 5,577 5 View

What is the best way for detection of biofilm production in staphylococci?

Hi i am trying to compere biofilm producing of different staphylococci isolates. But unfortunately with no luck. i followed this protocol: Cultures diluted 1:200 in TSB with 0.25% glucose and...

04 April 2020 8,209 2 View

Name of the plant active constituent which act as biofilm Disruptor by inhibiting Peptidyl-prolyl isomerase-B ??

natural Peptidyl-prolyl isomerase-B inhibitors

05 February 2020 6,505 1 View

I want to disrupt biofilm by using an electric current, what equipment can I use to achieve this?

I'm not sure how to achieve this in a microtitre plate or petri dish, I did think by electrophoresis, but is there anything else I can use and voltage?

31 December 2019 2,819 3 View