How to calculate refractive index (n and kvalue) against energy (in eV) data for alloyed nanomaterials through Independent Phase Approximation method?

Suggest me the method or some other software to calculate the above said data for nanoparticles.

21 February 2021 3,350 7 View

How can we make better contacts on thin films for I-V measurement?

Dear researchers, I am currently trying to perform I-V measurements for thin films. Could anybody let me know what is the best way to perform I-V measurements using Keithley 2600. How to make...

01 December 2020 9,103 9 View

How do I apply loading rate of 2.5 x 10^-5 mm/mm/sec in Abaqus?

I am trying to simulate the work carried out by Ilki et al. in 2008 on FRP Retrofit of Low and Medium Strength Circular and Rectangular Reinforced Concrete Columns. Within his research, the...

19 August 2020 2,755 4 View

Why my steel is not showing the RF values when being loaded in modelling of CFST on abaqus ?

I did the frequency analysis(selected buckle in linear perbutation ) , found the eigen value and gave the same as a load while again performing the analysis , this time selected general RICKS . I...

27 April 2020 6,536 13 View

I am looking for an open source seismic hazard and risk analysis software. Is there any software available free of cost?

Open source and easy to learn with proper documentation.

26 April 2020 4,994 2 View

In Phospharic Acid plant , Calendria is being used is generally of Graphite tube- shell & tube heat exchanger. Anyone using experience SS316 tubes?

Presently Majority of PA plants are using Graphite tubes in their system oh concentration for heat-exchange. Reliability is little less. We want to know that is ANY PA producing plants is having...

13 March 2020 4,877 3 View

Do we need a UDF for transient compressible simulation in Fluent?

I observed in some manuals and websites for performing transient simulation in Fluent that UDF (sinusoidal profile of pressure) has been used at the pressure outlet of the domain. Is it necessary...

31 October 2019 5,648 3 View

How much primer designing differs from probe designing for exome sequencing? What if we design biotynylated primer instead of probe for capturing?

What are the criteria for designing biotinylated probes differ from primer designing?

24 September 2019 8,205 2 View

Using continuos glucose monitoring system and glycemic index of foods?

Hello All, Can someone please share a protocol/ methodology for testing GI (glycemic index) of a food using CGMS (continuos glucose monitoring system). Is this kind of testing approved by FAO/...

26 August 2019 3,090 2 View

What accounts for absorption LSPR shifting to shorter wavelength regions over EM spectrum with increasing metal nanosphere size?

I have read about the absorption peak shifting to longer wavelength regions for semiconductors because with increasing size of the nanoparticle, the bandgap energy decreases and hence increases...

18 March 2019 7,085 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

No title

02 March 2021 3,060 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View