Beth Worley

4 Questions 2 Answers 0 Followers

Questions related from Beth Worley

Protocol for supplementing rodent chow with rosiglitazone (or other bioactive compound)?

Hello everyone, I'm planning a study that involves treating mice with the PPAR gamma agonist rosiglitazone. It seems like I have a few options for administering Rosi, including gavage, ip...

20 July 2022 1,003 2 View

Are there alternatives to Nitrogen drying for evaporating chloroform from lipids?

Hi everyone, I am following a protocol for extracting lipids from liver tissue for a cholesterol assay. The protocol calls for adding a 2:1 chloroform:methanol mixture, homogenizing, and rotating...

27 October 2015 3,581 15 View

Which breast cancer cell lines do you find grow best in nude mice?

I have worked exclusively with triple negative MDA-MB-231. We are thinking about redoing projects using a different TNBC cell line or ER+ line such as BT20 or MCF7, respectively. The xenograft...

08 July 2014 8,888 6 View

How long can fresh frozen tissue be stored at -80C?

I need to store fresh frozen tumor tissue from nude mice for western blotting, receptor binding assays, and immunohistochemistry. In our lab, we freeze tissue for western blots in RIPA buffer, in...

15 May 2014 1,562 9 View