Protocol for culturing primary neurons from adult pig brains?

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A paper on a fossil lycopod?

Dear all, Does anybody have a pdf of this paper? Wood, G.R. and Beeston, J.W., 1986. A late Permian lycopod cone Skilliostrobus sp. cf. S. australis Ash 1979 from Queensland. Geological Survey of...

07 August 2024 4,857 2 View

Dear colleagues, Can anybody by chance have pdfs of two books in Chinese: Liu (2003) and Zhang et al. (2004)?

Liu Zhaosheng. 2003. Triassic and Jurassic sporopollen assemblage from the Kuqa Depression，Tarim Basin of Xinjiang，NW China. Palaeontologia Sinica， New Series A，no. 14（Whole no. 190）....

28 July 2024 6,390 1 View

How to apply Kogut-Singh Index to measure cultural distance in intercultural dyads?

In my current study I'm measuring cultural diversity in intercultural coaching dyads by calculating cultural distance (Kogut-Singh Index, 1988) between coach and coachee in each intercultural...

03 June 2024 9,990 1 View

Как найти свой Researcher ID?

30 May 2024 6,369 0 View

How can I prepare electrospun webs for BET analysis?

How should I prepare electrospun fibers for BET analysis? How much (cm x cm) should I cut to obtain reliable results? What about degassing? I have PLA sample, 40 C will be enough? Thank you

23 April 2024 7,364 2 View

A pdf of Coquel & Brousmiche Delcambre (1996)?

Dear colleagues, Does anybody by chance have a pdf of this paper? Coquel, R., & Brousmiche Delcambre, C. (1996). Comparaisons spores in situ-spores dispersées chez quelques Equisétales,...

19 March 2024 8,258 3 View

How to represent qPCR data when a gene is not expressed in a group?

In my experiment, one group didn't have Cq when a use a specific primers to specific gene. But another groups was ok. How can i represent this results on the graph? I need to put Cq = 0 or Cq = 40?

17 March 2024 6,789 0 View

CD markers expression patterns?

Hi everyone, could you recommend any article on CD marker profile changes and their biological significance due to this change? I have been looking for something but can't find anything. In my...

10 March 2024 3,702 0 View

I can't see my purified PCR product in an agarose gel?

I successfully amplified fungal ITS from soil samples, however after running the purified PCR products in an agarose gel they are barely visible and don't look like defined bands but rather...

10 March 2024 8,377 2 View

LFP | Graphite pouch cell. Why is not discharging?

I'm stating to assembly LFP

14 January 2024 7,199 5 View

How to enrich pig excreta for increasing nutrient quality organically ?

Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.

09 August 2024 5,605 2 View

An animal in not a rock (e.g., Koch 2013), it has an internal constitution that can be modified?

Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...

07 August 2024 648 0 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

Are the GABAergic neurons electrophysiologically equal in all brain areas?

Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?

04 August 2024 4,090 0 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Standard curve of H2O2?

Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...

26 July 2024 3,460 2 View

Can primary care be sustainable ? And how ?

I am starting to work on this question, with particular reference to what we might be able to learn from countries outside the UK. Of course this topic is huge, and I am breaking sustainability...

25 July 2024 1,545 3 View

How to extract RNA from neurons?

Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...

23 July 2024 6,783 1 View

Best method for extracting RNA from morulae?

Best method and number of embryos for extracting RNA from pig morulae?

21 July 2024 4,398 1 View