Aldo Barrera Vásquez

6 Questions 7 Answers 0 Followers

Questions related from Aldo Barrera Vásquez

Protocol for Antibody Dissociation from Virus in Serum Samples?

Hello everyone, I'm currently working on a project where I need to isolate viruses in culture from incubation with PCR-positive serum samples, but I'm facing the challenge of dissociating...

18 June 2024 1,812 1 View

Does Promega's Passive Lysis 5X Buffer contains anti-protease and anti-phosphatase components?

Hi everyone, Do you know if the Passive Lysis 5X Buffer that Promega produce has these components? I always use it to lysate transfected cells and measure lucierase activity and now I'm wondering...

04 September 2018 1,467 1 View

Best way to induce responde against DNA damage in cell culture?

Hi!. I'm figuring out how to induce cellular stress by DNA damage. I'm working with HeLa and HEK 293T cells, and I need to study the effects of the CHK2 kinase pathway that phosphorylates a...

03 April 2018 7,550 3 View

How to design effective siRNAs against non coding regions of an mRNA?

Hi everyone. I'm working with the proteins hnRNP A1 and HuR (or ELAV-L1) and I need to silence the expression of the endogenous proteins to analyze the effect of over-expressed mutagenic...

04 October 2017 8,090 4 View

An efficient probe for the detection of HCV RNA?

Hi everyone, I want to design a protocol to detect viral RNA of Hepatitis C virus though real time PCR with probes. I used to use a regular qPCR with primers against the 5'UTR region (most...

06 January 2017 7,452 0 View

QRTPCR amplification of target with low GC content?

Hey everyone. I have a question. I'm doing the in vitro silencing of a small RNA of 79 bp (a snoRNA specifically), and I need to design a protocol for the detection of the known-down of this RNA....

27 December 2016 6,201 2 View