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Questions related from Aldo Barrera Vásquez
Hello everyone, I'm currently working on a project where I need to isolate viruses in culture from incubation with PCR-positive serum samples, but I'm facing the challenge of dissociating...
18 June 2024 1,921 1 View
Hi everyone, Do you know if the Passive Lysis 5X Buffer that Promega produce has these components? I always use it to lysate transfected cells and measure lucierase activity and now I'm wondering...
04 September 2018 1,560 1 View
Hi everyone! I'm trying to measure the cell cycle progression of HeLa cells, to complement other measurements involving cell grown. I know that the most used method is flow citometry, determining...
27 August 2018 473 4 View
Hi!. I'm figuring out how to induce cellular stress by DNA damage. I'm working with HeLa and HEK 293T cells, and I need to study the effects of the CHK2 kinase pathway that phosphorylates a...
03 April 2018 7,588 3 View
Hi everyone. I'm working with the proteins hnRNP A1 and HuR (or ELAV-L1) and I need to silence the expression of the endogenous proteins to analyze the effect of over-expressed mutagenic...
04 October 2017 8,130 4 View
Hi everyone, I want to design a protocol to detect viral RNA of Hepatitis C virus though real time PCR with probes. I used to use a regular qPCR with primers against the 5'UTR region (most...
06 January 2017 7,490 0 View
Hey everyone. I have a question. I'm doing the in vitro silencing of a small RNA of 79 bp (a snoRNA specifically), and I need to design a protocol for the detection of the known-down of this RNA....
27 December 2016 6,286 2 View
