Aldo Barrera Vásquez

4 Questions 6 Answers 0 Followers

Questions related from Aldo Barrera Vásquez

Does Promega's Passive Lysis 5X Buffer contains anti-protease and anti-phosphatase components?

Hi everyone, Do you know if the Passive Lysis 5X Buffer that Promega produce has these components? I always use it to lysate transfected cells and measure lucierase activity and now I'm wondering...

04 September 2018 1,445 1 View

How to design effective siRNAs against non coding regions of an mRNA?

Hi everyone. I'm working with the proteins hnRNP A1 and HuR (or ELAV-L1) and I need to silence the expression of the endogenous proteins to analyze the effect of over-expressed mutagenic...

04 October 2017 8,084 4 View

An efficient probe for the detection of HCV RNA?

Hi everyone, I want to design a protocol to detect viral RNA of Hepatitis C virus though real time PCR with probes. I used to use a regular qPCR with primers against the 5'UTR region (most...

06 January 2017 7,442 0 View

QRTPCR amplification of target with low GC content?

Hey everyone. I have a question. I'm doing the in vitro silencing of a small RNA of 79 bp (a snoRNA specifically), and I need to design a protocol for the detection of the known-down of this RNA....

27 December 2016 6,180 2 View