Suh Hee Cook

6 Questions 2 Answers 0 Followers

Questions related from Suh Hee Cook

Only middle wells of iPSC differentiate into cardiomyocytes?

Maintaining a WiCell fibroblast-derived line of iPSC in monolayer with laminin coating and E8 media, then using Wnti directed protocol (Gsk3i day 0, Wnti with conditioned media day 3, insulin day...

03 May 2024 6,270 1 View

Why won't my induced pluripotent stem cells form a pellet when passaging?

I culture human iPSC in monolayer for my research, kept in 6 well plates coated with either matrigel or laminin, and kept in either mtesr or iPSC brew (sometimes a 1:1 mixture). Previously they...

12 June 2023 5,444 1 View

Why don’t my iPSC form a cell pellet when centrifuged?

I culture human iPSC in monolayer for my research, kept in 6 well plates coated with either matrigel or laminin, and kept in either mtesr or iPSC brew (sometimes a 1:1 mixture). Previously they...

11 June 2023 8,803 1 View

Loss of confluency during iPSC culture?

I am culturing human induced pluripotent stem cells for differentiation into cardiomyocytes via the Wnti-directed protocol attached here. After passaging into a 12 well plate to start the...

23 February 2022 7,392 2 View

Protocol for administration of acetycholine and norepinherphrine to cells in in vitro culture?

Hello all! I work with induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) for my research. I am seeking to administer the positive chronotrope epinephrine and negative chronotrope...

30 June 2021 6,553 0 View

What type of dehumidifier should I use for an electrospinning system?

Hi, I'm a PhD student working on a cardiac tissue engineering project, and I'm setting up an electrospinning system to fabricate scaffolds. Any suggestions on what type of dehumidifier to buy? Do...

25 August 2020 9,838 6 View