Suh Hee Cook

3 Questions 1 Answers 0 Followers

Questions related from Suh Hee Cook

Why won't my induced pluripotent stem cells form a pellet when passaging?

I culture human iPSC in monolayer for my research, kept in 6 well plates coated with either matrigel or laminin, and kept in either mtesr or iPSC brew (sometimes a 1:1 mixture). Previously they...

12 June 2023 5,441 1 View

Loss of confluency during iPSC culture?

I am culturing human induced pluripotent stem cells for differentiation into cardiomyocytes via the Wnti-directed protocol attached here. After passaging into a 12 well plate to start the...

23 February 2022 7,390 2 View

Protocol for administration of acetycholine and norepinherphrine to cells in in vitro culture?

Hello all! I work with induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) for my research. I am seeking to administer the positive chronotrope epinephrine and negative chronotrope...

30 June 2021 6,504 0 View