VIF very high, but correlation is low and coefficient is significant. Which one whould I choose?

Hello. I have a question whether if my coefficients (statistically significant) would be valid when VIFs are really high. In my model, there are independent variables(IV), moderating variables...

26 February 2021 956 13 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Sb2Te3 thin film annealing and damage(break)?

21 January 2021 9,642 2 View

Can anyone advise me on how to amplify V4 region of fecal DNA of dss-induced colitic mice for microbiota profiling?

Animals were fed DSS via drinking water and fecal samples were collected and DNA was isolated using Qiagen stool DNA isolation kit. The V4 region of extracted DNA was amplified with specific...

20 December 2020 1,739 3 View

When comparing two very similar surveys taken over time, but with different sample size, what would be the best way to approach this problem?

Hi, I'm currently analysing a survey for a university course design, and some guidance would be very much appreciated. The students who are enrolled in a (year-long) course were given a link to...

08 December 2020 6,624 9 View

When analysing survey using Likert scale, is it okay to use Friedman's test?

Hi, I'm currently analysing a survey for a university course design, and some guidance would be very much appreciated. The students who are enrolled in a course were given a link to participate...

06 December 2020 6,060 2 View

Which time-serie model is good when using concatenate data?

I would like to make a model using sequence datasets. As I've searched that , many LSTM, RNN tutorials use long-sequential data such as weather in 1950-2020. However, my datasets are short...

24 November 2020 808 3 View

Could you answer some basic questions on CRISPR-cas9?

Hi, I would like to ask simple some questions related to CRISPR-Cas9 that I could not understand by reading some basic articles on it. I am not in that scientific field so my questions might be...

21 October 2020 2,439 2 View

How can I design resin column for test?

I'm interested in the research field of ion exchange resin. I want design about resin column with acryl for test. How do you usually design the diameter and length of small columns for test? I...

13 October 2020 3,443 2 View

How do you check the locations of brain-implanted cannulas?

Hello. I implanted single cannulas bilaterally into the brains of mice to infuse drugs. After behavior tests, what would be the simplese way to validate if the cannulas are in the target areas?...

18 September 2020 8,457 3 View

How can I prevent bubbles from forming under a coverslip?

My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...

10 February 2021 3,580 1 View

How can I remove bubble in WPU film?

I am trying to prepare water-based polyurethane films. When dried air bubbles were formed and destroys the film homogeneity, then I can't use this for mechanical test because of defects. Is there...

26 January 2021 701 1 View

How can I get rid of the bubbles in the SBS membrane?

I used methylcyclohexane/ ethylcyclohexane / n-butyl acetate mixed solvent to dissolve thermoplastic SBS & terpene resin. The mixture is then poured onto a glass substrate. After solvent...

24 January 2021 8,306 3 View

Can anyone provide me a protocl of Native PAGE with the 30% terminal resolving gel concentraion?

I am separating the AuCNs, which are Au nanoclusters. I used the 3% stacking and 30% resolving polyacrylamide from the reference. Eachtime when the resolving gel is polymerizing, plenty of heat...

22 January 2021 8,522 3 View

Any resist stripper which can remove thick metal based polymer residue and which is compatible with doped silicon ?

I am looking some resist strippers which can easily remove metal based polymer residue after post dry etch process. I have some candidates below but not sure if they are compatible with doped...

21 January 2021 3,008 3 View

Where can I find these two papers?

14 January 2021 1,572 3 View

How to model bubble coalescence and/or departure from a surface in ANSYS Fluent?

Dear all, I would like to simulate the pool boiling (subcooled). Specifically, I'm interested in departure of bubbles from finned surfaces. I want to do it in ANSYS-Fluent. Is it possible? if so,...

14 January 2021 6,304 5 View

Best way to get air out of a hamilton syringe?

I'm using a hamilton syringe to inject mineral oil through PVC tubing attached to the needle tip of the syringe. To make sure the tubing stays free of bubbles, I have to get all the air out of the...

10 January 2021 8,082 1 View

Why is PVDF splashing and bursting under vacuum ?

I prepared PVDF in a very regular way by dissolving it with stirring at room temperature but when i placed it in vacuum for eliminating the bubbles and trapped gases it started to burst and...

09 January 2021 3,965 3 View

What is the maximum time duration allowed between photoresist coating and exposure?

I have coated the Silicon wafer with photoresist (AZ1518) but until the exposure (Laser Writer) I had to wait for more than a week (Laser Writer was not operative) - would the photoresist still be...

03 January 2021 9,640 4 View