Which open source tools are suitable for proteome analysis?

I am looking for an open source tool to analyse proteomics. I have used STRING database so far. I want to compare to different data sets (two different conditions) and visualize differences in the...

15 July 2020 2,252 7 View

Does anyone have any experience with collecting SES from young children (e.g. 8-9 year olds)?

In combination with school performance of 8-9 year old children we would like to survey the SES (socioeconomic status). Normally this is done through a parents' questionnaire. But does anyone have...

24 June 2020 4,566 1 View

Image recognition using SVMs vs. CNNs?

I'm new in CNNs. After discovering the pre-formed deep CNNs, which can be used for feature extraction, I ask myself if there is still an application area in the field of image recognition, in...

11 February 2019 2,691 1 View

Is there any study on the fat content influencing the saltiness perceived?

I have tried to use products with same salt content but having different levels of fat. The products are bread , butter and cheese.

26 March 2016 5,214 3 View

Is it possible to have from 1 sample total LDH and RNA?

11 January 2016 2,484 2 View

Does anybody know why the OD of the corticosterone ELISA is high although the concentration is supposedly very low?

We perfomed a corticosterone ELISA from ENZO and our data are misleading. The sample we analysed contain only low concentrations but we detected an OD that hypothesized high values. I think there...

15 October 2015 9,538 6 View

How can I analyze a DGGE gel with Bionumerics?

I want to analyze my gels with Bionumerics and I tried the same things as they explained in the videos and in the quick guide, but it does not work. When I try to create a new comparison, I do not...

13 February 2015 6,854 7 View

What is an ideal housekeeping to use for proteins expression?

I am investigating a process called epithelial-mesenchymal transition (EMT) in which epithelial cells lose their typical characteristic and acquire mesenchymal markers. Cells undergo EMT...

08 November 2014 8,257 7 View

PCR to psa

I need to detect Pseudomonas syringae pv. actinidiae in symptomless plant material. What is the best PCR to do it, the PCR Rees-George et al. (2010) or Gallelli et al. (2011).I can only get one of...

21 May 2013 7,759 4 View

Enzimatic activity by marine microorganisms (extremophiles bacteria, isolated from deep sea)

Have someone get results from Bacillus indicus, a marine bacteria, isolated from 4000mts deep sea, for biosurfactant activity and production of lipase?? In the search I've made so far, none has...

01 January 1970 8,948 5 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View