Hi all:
I am working on a small (60a.a) archaeal protein. Scenario is:
Also, how can we predict protein function (if structure is known)?
What approaches can I take?
Thanks.
I don't find those two points contradictory at all, by producing more siderophores the strain is able to increase its access to Fe so can grow better at lower Fe concentrations. The WT strain can't get enough Fe when at low concentrations in the medium for good growth. You are thinking of siderophores as means to remove the Fe whereas in many cases they are a "transport" system to get the Fe to the cell.
Michael J. Benedik Thank you for clearing this point. I have just started my project on small proteins. So, quite new to protein work.
I suspect its an iron storage protein or siderophores operon repressor.
But i am not sure what approaches should i take to get to its actual function.
it could also be a sensor so that the cell things they are under iron deficiency. But it would seem to have a regularity function directly or indirectly.