I am processing immunoprecipitation with RAW 264.7 cell lysis. After protein assay, it was diluted with DW not IP buffer

(IP buffer = RIPA buffer + PBS + phosphatase inhibitor + protease inhibitor)

And it was inverted overnight in that condition. I am worried about whether that is okay to go on or not, because the A/G beads was expensive. If there is no use to go on I will do experiment again from cell lysis. There are not enough protein lysate to do IP.

Thank you.