Alternatives to thematic analysis for case study?

I am conducting a multiple-case study where I intend to compare a specific aspect between the two countries. When considering data analysis approaches, I came across TA several times but have not...

03 January 2021 8,166 3 View

Which AAV serotype is the best for SY5Y and HT22 cells?

I am not sure; is AAV2 best for SY5Y and HT22? Is there any comparative study for these lines? Also, Can i use same serotype vector for the two cell lines?

02 October 2020 3,328 2 View

Can i use ORI in my plasmid vector for replicating it in SH-SY5Y cells?

I want to use ORI in my plasmid vector for replicating it in SH-SY5Y cells like in 293E or 293T cells with SV40? Is there any ORI that can be replicated in SY5Y cells?

24 September 2020 2,541 3 View

Can be episomal plasmid vector amplified in mitotic cells?

Can be episomal plasmid vector amplified in mitotic cells without host- suitable ORI such as SV40 in mammalian cell that express sv40 large T antigen?

24 September 2020 1,570 4 View

How do you dissolve and inject indomethacin for rat studies?

I dissolved indomethacin (Santa Cruz Biotech - SC-200503) in DMSO and prepared stock of 25 mg/mL In my study, I inject 10 mg/kg i.p., injection volume 0,5 mL and I work with 250-300 g rats. When...

13 September 2020 5,659 3 View

Why do i get wrong total pressure data from Ansys Fluent?

Hi everyone, I have been working on a laminar compressible microtube flow analysis (supercritical fluid). Mass flow inlet, pressure outlet and constant wall heat flux boundary conditions are...

17 December 2019 1,403 4 View

Do you use the Cross-validation technique with the proposed model neural network and if yes do you use it after applying the model or before?

01 October 2019 6,959 3 View

How can cyclic symmetry be defined in dynamic, implicit analyse in Abaqus?

Hi, I need some support to define cyclic symmetry in dynamic, implicit analyse. As I have found out that cyclic symmetry under interactions is only usable in static, visco, frequency, steady...

26 November 2018 3,020 5 View

How do I find references to the size of the cDNA sequence I isolated?

How do I find references to the size of the cDNA sequence I isolated?

12 March 2018 2,101 3 View

Can be possible the dna binding domain's binding to two or more different DNA sequence in different enhancers?

Can be possible the transcription factor's dna binding domain's binding to two or more different DNA sequence in different(or also same) enhancers? Or there is no exception, it must be specific...

23 February 2018 8,240 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

How much should I centrifuge cells in agarose-coated plates to get spheroids?

Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.

09 February 2021 2,063 6 View