Prolyl endopeptidase enzyme activity

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Dominique Liger

Hi there,

The assay is based on the spectrophotometric properties of pNA: its absorbance will increase with the cleavage between proline and pNA (free pNA exhibit higher absorbing properties than pNA in the substrate peptide).

Belma Şenol

How is the activity of prolyl endopeptidase enzyme determined in the synthetic substrate Z-Gly-Pro-pNA? Are there any protocol recommendations?