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Questions related from Felipe Barros Teles
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
28 July 2024 6,543 0 View
Regarding the nomenclature of galactans found in agarophytes, which is the correct name for the polysaccharide: "agaran" or "agar"? Why is there so much confusion about the use of this term?
28 April 2024 4,564 3 View
Dear, In my experimental model I will administer a substance intraperitoneally and some time later I need to collect the T lymphocytes from the spleen for phenotypic characterization in order to...
26 November 2023 6,828 2 View
I will perform an immunoassay to detect the production of iNOS in macrophages by flow cytometry. I need to know what concentration of surfactant I should use to permeate the plasma membrane. Does...
19 August 2018 4,843 3 View
A buffer is composed of a weak acid and the corresponding conjugate base, so what is the meaning of a phosphate-citric acid buffer (McIlvaine buffer). In what situations is it used?
03 August 2017 473 2 View
In which macrophage receptor PLGA (poly(latic-co-glycolic acid) binds to be phagocytosed? I'm referring to the PLGA itself, it means: non-coated and non-loaded with any substance.
01 January 1970 4,273 3 View
What are the possibilities that a particle (range: 0.2 um - 10 um) has to enter in macrophages? I think they are: 1. Receptor-mediated Phagocytosis (classical); 2. Trigger Phagocitosys and 3. Non...
01 January 1970 5,440 1 View