Hi
I am using Fibrinogen (10mg/ml) and Thrombin (1000U/ml) to form fibrin clots. I found that after getting good cross linking initially the clots dissolve to liquid if left in incubator overnight. The two products are dissolved in DI water.
Anyone have any ideas why my cross linked gels are going into solution. This doesn't happen with Human Fibrinogen & Thrombin.