Johanna Prüller

3 Questions 4 Answers 0 Followers

Questions related from Johanna Prüller

Can CMV drive expression of multiple ORFs?

Hi, I have a plasmid where a tissue specific promoter drives my gene of interest followed by CMV driving expression of GFP. Is it theoretically possible that CMV is such a strong promoter, it can...

21 March 2019 3,577 1 View

SEM preparation of cell monolayer - can anyone help?

I want to prepare a monolayer of adherent cells grown on cover slips for SEM. I have previously only prepared gels and am wondering if I could use the same fixation/dehydration protocol for the...

03 November 2013 5,555 2 View

HSV-TK inhibits proliferation but doesn't kill cells

Hi, In my experiments I'm trying to induce apoptosis in cancer cells stably expressing HSV-TK by treating them with GCV. I observe a significant decrease in treated cells compared to control...

01 January 1970 494 1 View