I treated THP-1 with CU-CPT 4a (TLR3 inhibitors, MCE) and then co-cultures with bacteria for 72 hours to detected the expression of some interested genes. I repeated this assay for three time, and every time I obtained a totally different results.
Lucian Miles
Experimental setup – Can you confirm whether the THP-1 treatment method (e.g. cell line, drug concentration, incubation time, etc.) is based on published literature or validated protocols? Compound handling – Did you follow the recommended storage, stock solution, and working solution preparation on our website? Make sure the inhibitor is fully dissolved and properly stored before use.
Hi Rushuang Yan
Here are a few points that might help:
If you’re uncertain about any preparation steps, feel free to reach out to our technical support team [email protected] for more detailed guidance.
The answer to this question is provided by MedChemExpress Technical Support.
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