for last two months I try to culture WB-F344 cell which was purchased from JCRB cell bank. As mentioned in the documents I cultured them with MEM including 10% FBS, but after 4-6 passage the cells were died.
any suggestion ?
Tariq Khan
Use 20% fbs. And sont wait for 100% confluence, break cell at 60-70 confluency.
split flask frequentl. Some cell start die when more confluence.
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