I have experienced CD4 downregulation after PMA/iono stimulation and also TCR downregulation on PBLC. Does anybody have experience with TCR downregulation? I am trying to phenotype gd T cells in PBLC, but after PMA/iono/BFA stimulation TCRgd is almost gone, so I cannot gate on them. Anyone know how to avoid that? Is it due to PMA/iono or to Brefeldin A? Can anyone tell me about a good activation marker for PMA activated cells? (in order to maybe, titrate PMA until the lower effective concentration).

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