Maria J. Garcia-Leon

6 Questions 17 Answers 0 Followers

Questions related from Maria J. Garcia-Leon

How to analyse bioluminiscence images along time in mice?

I am a newby on in vivo imaging. We are using IVIS imaging system to study breast cancer metastasis in vivo, but we really do not know how to quantify the images along time. As the time pass, of...

25 December 2017 8,525 5 View

What software should I use to find common gene/s between several gene expression datasets?

I have not much experience in bioinformatics and I need to find what are the common genes in several gene expression datasets, in other words, I need to find genes that match in all (or some) of...

15 April 2014 2,815 2 View

How and where can I get the entire genome sequence of a miRNA?

I am quite new with miRNAs and I have a few basic questions: I am concerned about identifying the elements that drive the transcription of a miRNA precursor from a lentiviral plasmid. First of...

15 October 2013 9,693 21 View

Problem with CD4 and TCR downregulation after PMA/iono/BFA stimulation

I have experienced CD4 downregulation after PMA/iono stimulation and also TCR downregulation on PBLC. Does anybody have experience with TCR downregulation? I am trying to phenotype gd T cells in...

30 April 2013 7,182 15 View

Can anyone suggest a protocol for intracellular cytokine staining to stain ex vivo cells?

I want to know by FACS if a population of cells express several cytokines in basal conditions (no activation) within a given tissue. I directly get those cells ex-vivo from human tissue samples,...

04 February 2013 5,213 3 View

Can anyone suggest a method (other than Mander's coefficients) for measuring overlay/colocalization of two markers in immunohistological sections?

I want to quantify how many "green" cells also express a "red" marker. This seems to be easy to quantify by overlay (for example with the Mander's coefficient), but I am having a hard time because...

16 January 2013 6,188 3 View