Problem with Bruker's MALDI-TOF Biotyper Bacterial Standard.

More Praveen Rahi's questions See All

How to carry out survival analysis of a one time use machine which is stored and tested at regular intervals for functionality?

I want to carry out reliability study of a machine with various sub components both electrical and mechanical. The machine is a one time use (similar to a decoy or rocket) and is stored at...

30 June 2024 2,435 0 View

Offer or collaboration for book publications?

Dear Profs/ Scientist, if any one is interested or have plan to publish a good quality books/ book edited , I can contribute/help such type of research works . My research...

06 June 2024 5,932 2 View

Is there any home portable biowaste manager ?

home based bio waste management

02 June 2024 8,590 4 View

Call of book chapter ?

CALL FOR BOOK CHAPTERS (No Publication Fee) Scrivener Publishing/wiley Dear Prof,Scientist/ experts, Greetings. We are editing the book entitled " FET Devices: Post CMOS Theory and Applications...

28 May 2024 9,284 0 View

How to develop a flat panel display glasses ?

Dear friends, I am looking to develop a new project for the applications of displays using float glass. How and what process is needed to develop such flat panel displays what are the ideas and...

09 May 2024 3,233 0 View

How do we control pin holes in the coated thinfilms by using large area magnetron sputtering techniques?

Dear ResearchGate colleagues, professors, and scientists, I'm encountering challenges with coated thin films produced using a sputtering system. Following treatment through tempering at 650...

11 February 2024 3,585 4 View

Call of book chapters?

Dear Professors, Scientists, Researchers, Academicians, and Industry Professionals, Greetings of the day!!! We hope you are doing well! We would like to cordially invite you as chapter...

28 January 2024 194 0 View

PLGA Microspheres. Alcohol or ester terminated polymers?

I have read a research paper "Development of a novel morphological paclitaxel-loaded PLGA microspheres for effective cancer therapy: in vitro and in vivo evaluations". I want to synthesize PLGA...

13 December 2023 9,857 1 View

CALL FOR BOOK CHAPTERS (CRC Press): "Circuit Design Approach for Modern Applications"?

Dear Professors, Scientists, Researchers, Academicians, and Industry Professionals, Greetings of the day!!! We hope you are doing well! We would like to cordially invite you as chapter...

04 December 2023 7,273 2 View

Hello everyone, I am working on medicinal plant. How can i perform Principal components analysis?

I have done metabolite fingerprinting of plant extract using LC-MS and GC-MS.

29 November 2023 4,511 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this

05 August 2024 7,902 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Vivek Dogra

Dear Dr Rahi, you use 1% TFA or combination of 0.0.5%TFA (or FA) with 50-70% ACN

Praveen Rahi

Thanks vivek I got the answer ...:-). Actually it was not the issue of dissolution the standard got degraded...I was dissolving in the TFA and ACN sol...

Thanks Radu Tamaian, so you are also using MALDI TOF for microbial identifications.. or some other proteomics work.

That's great we have here autoflex speed with biotyper database and I am working in a culture collection.