I am using 4 chamber slides for seeding HEK-293T and HEK 293 and rat hypothema cell lines. I am using RPMI-1640 from SIGMA with 10% FBS, 1% Gutamax and P/S.
seeding cells also on 24 wells (1x10*5) the same as chamber slides.
The problem is cells gathered in the center of chmaber slides and after trasnfection, they are floating more than 60 % like a cloud of cells.
I also tried with DMEM-H but I found the same problem.
I would be please of any kind of suggestion or help to sole this problem