Respected/Dear Researchers
Can anyone suggest me, the easy way of astaxanthin production from Haematococcus pluvialis. There is no astaxanthin production were observed one month old culture.
1. The strain is pure
2. Temp. 20ºC
3. Nitrogen depletion method were tried (Conways, Boldbasal medium).
Questions?
1. What stress I need to try?
2. Temp and medium?
Thanks in advance.
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Try pH stress, phosphate stress, light stress (high intensity and low intensity) and nitrogen stress as well.
Dear Kumar,
I think you could try to apply inorganic carbon sources to the culture, i.e. sodium bicarbonate (you will need to adjust pH).
Good luck with your work,
Davide
Did you try salt stress? temp 25ºC and pH 7.
Make sure that you are using H. pluvialis.
I suggest you follow up your culture until it gets stationary, after which you can wash it with medium devoid of nitrate or phosphate and increase your light intensity >100µmol photons.m².s-1
you can increase the light intensity and or add acetic acid
Hello i have the same problem as well i m working in the production of astaxanthin from haematococcus ,i m working with 4 strains of haematococcus one is purchased from a society and others i isolated them
I made the culture in BBM medium to monitore growth and after i wanted to stressed them it s not working they are all time in vegetative stage i tried many sort of stress factors by light deprivation of medium (N,P,and both) it s not working at all i don t know where is the problem if you could help me guys i will be so gratful
Best regards.
Hey everybody i had the same problem with my culture and finally it works well i was able to stress my microlagae, here some advices for you ,firstly you should make sure that you are working with a pure strain, secondly try stress with salinity and acetate combined with high light intensity as it mentioned in many articles and it s gonna work hope that my answer will help you.
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