Does using a likert scale in my qualitative research will make it mixed method?

I am conducting a qualitative research and using two qualitative data collection tools (1. Reflective journal 2. Semi-structured interviews). In the reflective journal I have asked the...

10 December 2020 4,672 7 View

How to analyze DPPH antioxidant scavenging of AgNPs synthesized by natural methanolic extract?

How to make stock solution which diluted into various concentration (ex. 10, 20, 30 ppm) and added by DPPH solution. Then read by spectrophotometer UV-Vis? Should I sentrifuge the solution of...

10 December 2019 8,354 5 View

In TEM sample preparation what quantity of nanoparticle needs to be mixed in ethanol before ultra-sonication? min quantity that gives results?

I have to send sample in another city for TEM analysis, they said send prepared sample..

25 June 2019 5,113 3 View

How to simply fabricate homogenous boron nitride film on the slide glass?

Hello, i have a question regarding on surface fabrication technique, since i am not familiar with this work before. I am going to try to fabricate a thin boron nitride (BN) film on the bare glass...

03 December 2018 3,406 3 View

What is the minimum concentration of methanol extract of plant non-toxic to human cell-line? Should I use the extract and bacteria at the same time?

I am testing effect of plant extract on human cell line (hacat) infected with bacteria. My extract is prepared in methanol (10 g of plant in 100ml of methanol). What concentration of extract...

03 December 2018 2,169 3 View

Can anyone help me with the PLA blends thin film

I want to produce PLA blends by using solution casting method. I am using chloroform as a solvent. The steps are conducted in fume chamber. I have precondition the PLA in an oven at 70 deg around...

13 August 2017 5,987 2 View

Can restriction enzymes only be added to the end of a primer or is it possible to add it within the primer sequence?

Testing primers previously designed where restriction enzymes were located in the middle of the primer sequence but not sure whether this is actually possible.

02 May 2017 2,969 3 View

Could somebody help me about small scale endophyte fermentation ?

I have read some methods to start endophyte fermentation. Some using starter, and some are not. I wanna ask three question 1. how important is starter for small scale fermentation (100 mL) ? 2....

09 October 2016 7,720 10 View

When doing antioxidant assays (plants), would one need to generate a standard curve for the specific assay?

I will be doing the DPPH. SOD, CAT , AP and GR assays and was wondering how I'd determine if there is any effect when comparing my plant samples subjected to different abiotic and biotic stresses.

17 March 2015 4,066 5 View

How does to calculate treatment time for Cobalt-60 teletherapy unit in SAD/isocentric technique?

Treatment time (for teleterapy unit) is similar with monitor unit (for LINAC). Manual calculation for MU or time depends on dose delivery technique, there are SSD tech. and isocentric tech. To...

25 December 2014 3,099 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View