Primer and Probe designing for DNA methylation studies?

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How to quantify promoter DNA methylation?

I am using MethyLight assay to quantify promoter DNA methylation in tumor tissues. For a standard curve, I am using serial dilution of methylated standard with unmethylated DNA. As I followed with...

08 September 2013 7,147 16 View

How does methylated cytosine resist bisulphite modification to uracil?

How does a methylated cytosine resists bisulphite treatment whereas unmethylated cytosines are converted into uracil?

06 July 2013 6,395 1 View

How does one prepare nuclease free water?

I am trying to isolate mRNA for ovarian tumor tissue. The protocol requires a lot of sterile nuclease free water. Can anyone suggest to me how to prepare sterile nuclease free water in lab, as...

05 June 2013 965 11 View

How to estimate DNA methylation using real time PCR

I am trying to quantitate promoter methylation of six tumor suppressor genes in ovarian cancer tissue sample. Can anyone suggest which is the best method for quantitation of DNA promoter...

01 February 2013 9,044 18 View

Conventional PCR or Real Time PCR for a Loss of Heterozygosity analysis?

I am planning to do a loss of heterozygosity (LOH) analysis for the CDH13 gene at different polymorphic microsatellite markers in ovarian tumor tissue. Can anyone suggest which is the better...

11 December 2012 6,229 16 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Maryam Mortezaeefar

Genome-wide analysis of DNA methylation patterns., doi:10.1242/dev.001131

Detection of DNA methylation in eucaryotic cells., A. Sulewska et al

Uyen Le Thanh Nha

you can try the Methyl Primer Express software. It's free and available at website:http://www.appliedbiosystems.com/absite/us/en/home/support/software-community/free-ab-software.html