Rahul Bhagat

6 Questions 7 Answers 0 Followers

Questions related from Rahul Bhagat

How to quantify promoter DNA methylation?

I am using MethyLight assay to quantify promoter DNA methylation in tumor tissues. For a standard curve, I am using serial dilution of methylated standard with unmethylated DNA. As I followed with...

09 September 2013 7,170 16 View

How does methylated cytosine resist bisulphite modification to uracil?

How does a methylated cytosine resists bisulphite treatment whereas unmethylated cytosines are converted into uracil?

07 July 2013 6,414 1 View

How does one prepare nuclease free water?

I am trying to isolate mRNA for ovarian tumor tissue. The protocol requires a lot of sterile nuclease free water. Can anyone suggest to me how to prepare sterile nuclease free water in lab, as...

06 June 2013 986 11 View

How to estimate DNA methylation using real time PCR

I am trying to quantitate promoter methylation of six tumor suppressor genes in ovarian cancer tissue sample. Can anyone suggest which is the best method for quantitation of DNA promoter...

02 February 2013 9,068 18 View

Primer and Probe designing for DNA methylation studies?

How to design Real time PCR primer and probe for DNA methylation studies?

12 December 2012 1,153 4 View

Conventional PCR or Real Time PCR for a Loss of Heterozygosity analysis?

I am planning to do a loss of heterozygosity (LOH) analysis for the CDH13 gene at different polymorphic microsatellite markers in ovarian tumor tissue. Can anyone suggest which is the better...

12 December 2012 6,258 16 View